| RRC ID |
18987
|
| Author |
Feng GG, Li C, Huang L, Tsunekawa K, Sato Y, Fujiwara Y, Komatsu T, Honda T, Fan JH, Goto H, Koide T, Hasegawa T, Ishikawa N.
|
| Title |
Naofen, a novel WD40-repeat protein, mediates spontaneous and tumor necrosis factor-induced apoptosis.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Naofen has recently been identified from the rat brain/spinal cord cDNA library as a substance reactive against an anti-shigatoxin (Stx)-2 antibody. Naofen mRNA is composed of 4620 nucleotides and encodes 1170 amino acids. Naofen contains four WD-repeat domains in its N-terminus and is ubiquitously distributed in many tissues of the rat. Tumor necrosis factor (TNF)-alpha enhanced the expression of naofen mRNA in HEK293 cells in a dose-dependent manner. Furthermore, naofen siRNA, which predominantly knocked down the expression of naofen mRNA, significantly reduced both TNF-alpha-induced caspase-3 activation and apoptosis in HEK293 cells. Overexpression of naofen in HEK293 cells (FLAG-NF) spontaneously induced caspase -3 activation and apoptosis, and showed extremely high susceptibility to TNF-alpha-induced apoptosis. These results indicated that naofen may function as a novel modulator activating caspase-3, and promoting TNF-alpha-stimulated apoptosis.
|
| Volume |
394(1)
|
| Pages |
153-7
|
| Published |
2010-3-26
|
| DOI |
10.1016/j.bbrc.2010.02.133
|
| PII |
S0006-291X(10)00367-0
|
| PMID |
20193664
|
| MeSH |
Animals
Apoptosis*
Apoptosis Regulatory Proteins / genetics
Apoptosis Regulatory Proteins / metabolism*
Caspase 3 / biosynthesis
Cell Line
Enzyme Activation
Humans
Proteins / genetics
Proteins / metabolism*
RNA, Messenger / metabolism
Rats
Rats, Wistar
Tissue Distribution
Tumor Necrosis Factor-alpha / pharmacology
Tumor Necrosis Factor-alpha / physiology
|
| IF |
2.985
|
| Times Cited |
10
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
293(RCB1637) |
