Reference - Detail
| RRC ID | 18991 |
|---|---|
| Author | Michikawa Y, Suga T, Ishikawa A, Hayashi H, Oka A, Inoko H, Iwakawa M, Imai T. |
| Title | Genome wide screen identifies microsatellite markers associated with acute adverse effects following radiotherapy in cancer patients. |
| Journal | BMC Med Genet |
| Abstract |
BACKGROUND:The response of normal tissues in cancer patients undergoing radiotherapy varies, possibly due to genetic differences underlying variation in radiosensitivity. METHODS:Cancer patients (n = 360) were selected retrospectively from the RadGenomics project. Adverse effects within 3 months of radiotherapy completion were graded using the National Cancer Institute Common Toxicity Criteria; high grade group were grade 3 or more (n = 180), low grade group were grade 1 or less (n = 180). Pooled genomic DNA (gDNA) (n = 90 from each group) was screened using 23,244 microsatellites. Markers with different inter-group frequencies (Fisher exact test P < 0.05) were analyzed using the remaining pooled gDNA. Silencing RNA treatment was performed in cultured normal human skin fibroblasts. RESULTS:Forty-seven markers had positive association values; including one in the SEMA3A promoter region (P = 1.24 x 10(-5)). SEMA3A knockdown enhanced radiation resistance. CONCLUSIONS:This study identified 47 putative radiosensitivity markers, and suggested a role for SEMA3A in radiosensitivity. |
| Volume | 11 |
| Pages | 123 |
| Published | 2010-8-11 |
| DOI | 10.1186/1471-2350-11-123 |
| PII | 1471-2350-11-123 |
| PMID | 20701746 |
| PMC | PMC2928773 |
| MeSH | Fibroblasts Humans Microsatellite Repeats Neoplasms / genetics Neoplasms / radiotherapy* Radiation Tolerance / genetics* |
| IF | 1.585 |
| Times Cited | 12 |
| WOS Category | GENETICS & HEREDITY |
| Resource | |
| Human and Animal Cells | NB1RGB(RCB0222) |