RRC ID 19066
Author Puramattathu TV, Islam MR, Nishie M, Yanagihara S, Nagao J, Okuda K, Zendo T, Nakayama J, Sonomoto K.
Title Enhanced production of nukacin D13E in Lactococcus lactis NZ9000 by the additional expression of immunity genes.
Journal Appl. Microbiol. Biotechnol.
Abstract Nukacin D13E (D13E) is a variant of type-A(II) lantibiotic nukacin ISK-1 produced by Staphylococcus warneri ISK-1. D13E exhibited a twofold higher specific antimicrobial activity than nukacin ISK-1 against a number of Gram-positive bacteria. We previously reported the heterologous production of D13E in Lactococcus lactis NZ9000 under the control of nisin-controlled gene expression system. In this study, we demonstrated enhanced production of D13E by the additional expression of immunity genes, nukFEG. The nukacin ISK-1 immunity, conferred by the ABC transporter complex, NukFEG, and the lantibiotic-binding protein, NukH, was not overwhelmed by D13E. The additional NukFEG resulted in a fourfold increase in the immunity level of the strain and a 5.2-fold increase in D13E production. The additional NukFEGH-expressing strain with the highest D13E immunity showed reduced level of production. Further improvement in D13E production was achieved by using pH-controlled batch fermentation.
Volume 93(2)
Pages 671-8
Published 2012-1
DOI 10.1007/s00253-011-3563-1
PMID 21904816
MeSH Bacteriocins / genetics Bacteriocins / metabolism* Biological Transport Drug Resistance, Bacterial* Gene Expression Lactococcus lactis / genetics Lactococcus lactis / metabolism* Membrane Transport Proteins / genetics Membrane Transport Proteins / metabolism Mutant Proteins / genetics Mutant Proteins / metabolism Mutation, Missense Recombinant Proteins / genetics Recombinant Proteins / metabolism
IF 3.34
Times Cited 2
General Microbes JCM 1157 JCM 11004