RRC ID 1910
著者 Sugimoto M, Fujikawa A, Womack JE, Sugimoto Y.
タイトル Evidence that bovine forebrain embryonic zinc finger-like gene influences immune response associated with mastitis resistance.
ジャーナル Proc Natl Acad Sci U S A
Abstract Mastitis, a mammary gland inflammation in response to bacterial infection, is a major problem in the dairy industry. We found that cows susceptible to mastitis have a three-base insertion in a glycine-coding stretch of the gene for forebrain embryonic zinc finger-like (FEZL), a transcription factor with a role in neuronal development. Mastitis induces FEZL expression in mammary glands, and induced FEZL promotes expression of the axon-attracting molecule semaphorin 5A (SEMA5A) through a GCAG sequence. FEZL also induces SEMA5A expression in susceptible cattle but at a lower level than in resistant cattle. Enhanced SEMA5A induces expression of at least nine genes related to the host's immune response, including TNF-alpha and IL-8. We propose that susceptibility to mastitis results from an impaired immune response due to the lower transcription activity of susceptible FEZL. Our results provide an avenue to select for genetic improvement of resistance to mastitis and suggest that the FEZL-SEMA5A pathway might control both neuronal development and innate immunity.
巻・号 103(17)
ページ 6454-9
公開日 2006-4-25
DOI 10.1073/pnas.0601015103
PII 0601015103
PMID 16611727
PMC PMC1458905
MeSH Adaptor Proteins, Vesicular Transport / genetics Adaptor Proteins, Vesicular Transport / immunology Adaptor Proteins, Vesicular Transport / metabolism Animals Base Sequence Binding Sites / genetics Cattle Cell Line DNA / genetics DNA / metabolism Female Gene Expression Genetic Linkage Haplotypes Interleukin-8 / biosynthesis Mastitis, Bovine / genetics* Mastitis, Bovine / immunology* Mastitis, Bovine / metabolism Molecular Sequence Data Nerve Tissue Proteins / genetics* Nerve Tissue Proteins / immunology* Nerve Tissue Proteins / metabolism Prosencephalon / embryology Prosencephalon / immunology Receptors, Immunologic / genetics Receptors, Immunologic / metabolism Recombinant Proteins / genetics Recombinant Proteins / immunology Recombinant Proteins / metabolism Signal Transduction Transcription Factors / genetics* Transcription Factors / immunology* Transcription Factors / metabolism Transfection Tumor Necrosis Factor-alpha / biosynthesis Zinc Fingers / genetics* Zinc Fingers / immunology*
IF 9.412
引用数 45
WOS 分野 GENETICS & HEREDITY
リソース情報
ヒト・動物細胞 OCUB-M(RCB0881)