論文 - 詳細
| RRC ID | 19114 |
|---|---|
| 著者 | Irie K, Shimomura T, Fujiyoshi Y. |
| タイトル | The C-terminal helical bundle of the tetrameric prokaryotic sodium channel accelerates the inactivation rate. |
| ジャーナル | Nat Commun |
| Abstract |
Most tetrameric channels have cytosolic domains to regulate their functions, including channel inactivation. Here we show that the cytosolic C-terminal region of NavSulP, a prokaryotic voltage-gated sodium channel cloned from Sulfitobacter pontiacus, accelerates channel inactivation. The crystal structure of the C-terminal region of NavSulP grafted into the C-terminus of a NaK channel revealed that the NavSulP C-terminal region forms a four-helix bundle. Point mutations of the residues involved in the intersubunit interactions of the four-helix bundle destabilized the tetramer of the channel and reduced the inactivation rate. The four-helix bundle was directly connected to the inner helix of the pore domain, and a mutation increasing the rigidity of the inner helix also reduced the inactivation rate. These findings suggest that the NavSulP four-helix bundle has important roles not only in stabilizing the tetramer, but also in accelerating the inactivation rate, through promotion of the conformational change of the inner helix. |
| 巻・号 | 3 |
| ページ | 793 |
| 公開日 | 2012-4-24 |
| DOI | 10.1038/ncomms1797 |
| PII | ncomms1797 |
| PMID | 22531178 |
| PMC | PMC3337986 |
| MeSH | Amino Acid Motifs Amino Acid Sequence Bacterial Proteins / chemistry* Bacterial Proteins / genetics Bacterial Proteins / metabolism* Crystallography, X-Ray Kinetics Models, Molecular Molecular Sequence Data Phylogeny Point Mutation Protein Multimerization Protein Structure, Tertiary Rhodobacteraceae / chemistry Rhodobacteraceae / classification Rhodobacteraceae / genetics Rhodobacteraceae / metabolism* Sodium Channels / chemistry* Sodium Channels / genetics Sodium Channels / metabolism* |
| IF | 12.121 |
| 引用数 | 17 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 21789 |