RRC ID 19134
著者 Iwaki T, Fujita Y, Tanaka N, Giga-Hama Y, Takegawa K.
タイトル Mitochondrial ABC transporter Atm1p is required for protection against oxidative stress and vacuolar functions in Schizosaccharomyces pombe.
ジャーナル Biosci Biotechnol Biochem
Abstract A potential correlation between mitochondrial and vacuolar functions is known to exit in yeast. Fission yeast atm1(+), SPAC15A10.01, encodes a putative half-type ABC transporter with an N-terminal mitochondrial-targeting signal. In an attempt to evaluate the possible involvement of mitochondrion in vacuole function, a functional analysis of atm1(+) was performed by gene disruption. Growth of the atm1 mutant was inhibited in the presence of oxidizing agents, and S. cerevisiae Atm1p was found to complement this growth defect. atm1Delta cells exhibited defects in fluid-phase endocytosis and vacuolar fusion under hypotonic stress. GFP-tagged Atm1p was observed to be localized in the mitochondria. These data strongly suggest that fission yeast Atm1p was not only involved in protection against oxidative stress, but also played a role in vacuolar functions.
巻・号 69(11)
ページ 2109-16
公開日 2005-11-1
DOI 10.1271/bbb.69.2109
PII JST.JSTAGE/bbb/69.2109
PMID 16306692
MeSH ATP-Binding Cassette Transporters / genetics ATP-Binding Cassette Transporters / physiology* Endocytosis / genetics Mitochondria / physiology Mitochondrial Proteins / physiology* Oxidants / pharmacology Oxidative Stress* Saccharomyces cerevisiae Proteins / physiology* Schizosaccharomyces / genetics Schizosaccharomyces / growth & development Schizosaccharomyces pombe Proteins / physiology* Vacuoles / physiology*
IF 1.516
引用数 6
WOS 分野 CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
酵母 FY17330(YF16) FYP466(pTN197)