RRC ID 19167
著者 Rico P, González-García C, Petrie TA, García AJ, Salmerón-Sánchez M.
タイトル Molecular assembly and biological activity of a recombinant fragment of fibronectin (FNIII(7-10)) on poly(ethyl acrylate).
ジャーナル Colloids Surf B Biointerfaces
Abstract Fibronectin (FN) fibrillogenesis is a cell-mediated process involving integrin activation that results in conformational changes of FN molecules and the organization of actin cytoskeleton. A similar process can be induced by some particular chemistries in the absence of cells, e.g., poly(ethyl acrylate) (PEA), which enhance FN-FN interactions leading to the formation of a biologically active network on the material surface. We have investigated the organization of a recombinant fragment of fibronectin (FNIII(7-10)) upon adsorption on this particular chemistry, PEA. Atomic force microscopy (AFM) was used to identify individual molecules of the fragment after adsorption, as well as the evolution of the distribution of adsorbed molecules on the surface of the material as the concentration of the adsorbing solution increased. Globular molecules that turn into small aggregates were found as a function of solution concentration. Above a threshold concentration of the adsorbing solution (50 microg/mL) an interconnected network of the FNIII(7-10) fragment is obtained on the material surface. The bioavailability of specific cell adhesion domains, including RGD, within the molecules was higher on PEA than on the control glass. The biological activity of the fragment was further investigated by evaluating focal adhesion formation and actin cytoskeleton for MC3T3-E1 osteoblast-like cells. Well-developed focal adhesion complexes and insertions of actin stress fibers were found on PEA in a similar way as it happens in the control SAM-OH. Moreover, increasing the hydrophilicity of the surface by incorporating -OH groups led to globular molecules of the fragment homogeneously distributed throughout the surface; and the cell-material interaction is reduced as depicted by the lack of well-developed focal plaques and actin cytoskeleton.
巻・号 78(2)
ページ 310-6
公開日 2010-7-1
DOI 10.1016/j.colsurfb.2010.03.019
PII S0927-7765(10)00167-0
PMID 20409696
MeSH 3T3 Cells Acrylic Resins / chemistry* Acrylic Resins / metabolism Actins / chemistry Actins / metabolism Adsorption Animals Binding Sites Cell Adhesion Coated Materials, Biocompatible / chemistry Coated Materials, Biocompatible / metabolism Fibronectins / chemistry* Fibronectins / genetics Fibronectins / metabolism Fluorescent Antibody Technique Focal Adhesions Hydrophobic and Hydrophilic Interactions Mice Microscopy, Atomic Force Peptide Fragments / chemistry* Peptide Fragments / genetics Peptide Fragments / metabolism Recombinant Proteins / chemistry Recombinant Proteins / metabolism Recombinant Proteins / pharmacokinetics Surface Properties Vinculin / chemistry Vinculin / metabolism
IF 4.389
引用数 12
WOS 分野 BIOPHYSICS CHEMISTRY, PHYSICAL MATERIALS SCIENCE, BIOMATERIALS
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)