RRC ID 19177
Author Harada M, Kubo H, Abe T, Maezawa H, Otsuka H.
Title Selection of endogenous 13C substrates for observation of intracellular metabolism using the dynamic nuclear polarization technique.
Journal Jpn J Radiol
Abstract PURPOSE:The aim of this study was to select a suitable substrate candidate for dynamic nuclear polarization (DNP) studies and demonstrate its utility for evaluating intracellular metabolism.
MATERIALS AND METHODS:Hyperpolarized substances included 1-(13)C-pyruvate (Pyr), 1-(13)C-glucose (Glc), and 1-(13)C-acetate. A DNP polarizer and a 600-MHz vertical small-bore scanner were used for (13)C-MR spectroscopic measurements. After polarization for 1 h, the dissolved solution was injected via a capillary line into the nuclear magnetic resonance tube in the scanner. The sequential spectra of the hyperpolarized (13)C-labeled substrates were acquired in durations of more than 120 s, and a thermal spectrum was obtained more than 1 h thereafter. FM3A cancer cells of mammary tumors were cultured for intracellular detection of the hyperpolarized (13)C-substances.
RESULTS:The greatest sensitivity was found using Pyr with the longest T1 decay (51.5 s); and remarkably, the least sensitivity was observed using Glc with a signal decay of less than 2 s. An effective increase in sensitivity was shown using the other substances. The hyperpolarized intracellular study using (13)C-Pyr showed distinct elevation of lactate levels.
CONCLUSION:The DNP technique is useful for evaluating intracellular metabolism. However, Glc is not suitable for use with the DNP technique.
Volume 28(2)
Pages 173-9
Published 2010-2-1
DOI 10.1007/s11604-009-0390-8
PMID 20182855
MeSH Acetates / metabolism* Animals Biomarkers, Tumor / metabolism Carbon Isotopes / metabolism* Cell Line, Tumor / metabolism Cells, Cultured Female Glucose / metabolism* Image Enhancement / methods Magnetic Resonance Spectroscopy / methods* Mammary Neoplasms, Experimental / metabolism* Mice Mice, Inbred C3H Pyruvic Acid / metabolism* Sensitivity and Specificity
IF 1.439
Times Cited 13
Human and Animal Cells FM3A(RCB0086)