論文 - 詳細
| RRC ID | 19189 |
|---|---|
| 著者 | Wakao S, Hayashi T, Kitada M, Kohama M, Matsue D, Teramoto N, Ose T, Itokazu Y, Koshino K, Watabe H, Iida H, Takamoto T, Tabata Y, Dezawa M. |
| タイトル | Long-term observation of auto-cell transplantation in non-human primate reveals safety and efficiency of bone marrow stromal cell-derived Schwann cells in peripheral nerve regeneration. |
| ジャーナル | Exp Neurol |
| Abstract |
Based on their differentiation ability, bone marrow stromal cells (MSCs) are a good source for cell therapy. Using a cynomolgus monkey peripheral nervous system injury model, we examined the safety and efficacy of Schwann cells induced from MSCs as a source for auto-cell transplantation therapy in nerve injury. Serial treatment of monkey MSCs with reducing agents and cytokines induced their differentiation into cells with Schwann cell properties at a very high ratio. Expression of Schwann cell markers was confirmed by both immunocytochemistry and reverse transcription-polymerase chain reaction. Induced Schwann cells were used for auto-cell transplantation into the median nerve and followed-up for 1year. No abnormalities were observed in general conditions. Ki67-immunostaining revealed no sign of massive proliferation inside the grafted tube. Furthermore, (18)F-fluorodeoxygluocose-positron emission tomography scanning demonstrated no abnormal accumulation of radioactivity except in regions with expected physiologic accumulation. Restoration of the transplanted nerve was corroborated by behavior analysis, electrophysiology and histological evaluation. Our results suggest that auto-cell transplantation therapy using MSC-derived Schwann cells is safe and effective for accelerating the regeneration of transected axons and for functional recovery of injured nerves. The practical advantages of MSCs are expected to make this system applicable for spinal cord injury and other neurotrauma or myelin disorders where the acceleration of regeneration is expected to enhance functional recovery. |
| 巻・号 | 223(2) |
| ページ | 537-47 |
| 公開日 | 2010-6-1 |
| DOI | 10.1016/j.expneurol.2010.01.022 |
| PII | S0014-4886(10)00042-7 |
| PMID | 20153320 |
| MeSH | Animals Bone Marrow Cells / cytology Bone Marrow Transplantation / adverse effects Bone Marrow Transplantation / methods* Cell Differentiation / physiology Cell Division / physiology Cells, Cultured Disease Models, Animal Fluorodeoxyglucose F18 Macaca fascicularis Male Median Nerve / diagnostic imaging Median Nerve / injuries Median Nerve / pathology Median Neuropathy / diagnostic imaging Median Neuropathy / pathology Median Neuropathy / therapy* Motor Neurons / cytology Motor Neurons / physiology Motor Skills / physiology Nerve Regeneration / physiology* Neural Conduction / physiology Positron-Emission Tomography Recovery of Function / physiology Schwann Cells / cytology Schwann Cells / transplantation* Stromal Cells / cytology* Time Factors Transplantation, Autologous |
| IF | 4.691 |
| 引用数 | 76 |
| WOS 分野 | NEUROSCIENCES |
| リソース情報 | |
| ヒト・動物細胞 | YST-1(RCB2136) |