| RRC ID |
1919
|
| 著者 |
Obama K, Ura K, Satoh S, Nakamura Y, Furukawa Y.
|
| タイトル |
Up-regulation of PSF2, a member of the GINS multiprotein complex, in intrahepatic cholangiocarcinoma.
|
| ジャーナル |
Oncol Rep
|
| Abstract |
To disclose molecular mechanisms of cholangiocarcinogenesis and to search for novel diagnostic markers and therapeutic targets for cholangiocarcinoma, we previously analyzed gene-expression profiles of 25 intrahepatic cholangiocarcinomas (ICCs) by means of a cDNA microarray re-presenting 27,648 genes. Among the genes frequently up-regulated in the cancer cells, we focused on PSF2 (partner of SLD five 2), a component of the GINS multiprotein complex that plays a crucial role in initiation of DNA replication. Semi-quantitative RT-PCR analysis of clinical samples confirmed high levels of PSF2 expression in the cancer cells, but expression of this gene was barely detectable in normal vital organs. Transfection of ETK-1 and HuH28 cells with short-interfering RNA specific to PSF2 reduced the amount of transcript and suppressed cell growth, suggesting that PSF2 may play an important role in development of cholangio-carcinoma. The findings reported here provide new insights into human cholangiocarcinogenesis and may contribute to the development of novel therapeutic drugs for this type of liver cancer.
|
| 巻・号 |
14(3)
|
| ページ |
701-6
|
| 公開日 |
2005-9-1
|
| PMID |
16077978
|
| MeSH |
ATP Binding Cassette Transporter, Subfamily B, Member 2
ATP Binding Cassette Transporter, Subfamily B, Member 3
ATP-Binding Cassette Transporters / genetics*
Animals
Bile Duct Neoplasms / genetics
Bile Duct Neoplasms / pathology*
Bile Ducts, Intrahepatic / metabolism
Bile Ducts, Intrahepatic / pathology*
Cell Line, Tumor
Cell Proliferation
Cells, Cultured
Cholangiocarcinoma / genetics
Cholangiocarcinoma / pathology*
DNA-Binding Proteins / genetics
Female
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
Male
Mice
NIH 3T3 Cells
Plasmids / genetics
RNA Interference / physiology
RNA, Small Interfering / genetics
RNA, Small Interfering / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Transfection
Up-Regulation / genetics
|
| IF |
3.417
|
| 引用数 |
37
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
SSP-25(RCB1293)
RBE(RCB1292)
ETK-1(RCB1289) |
