論文 - 詳細
| RRC ID | 1919 |
|---|---|
| 著者 | Obama K, Ura K, Satoh S, Nakamura Y, Furukawa Y. |
| タイトル | Up-regulation of PSF2, a member of the GINS multiprotein complex, in intrahepatic cholangiocarcinoma. |
| ジャーナル | Oncol Rep |
| Abstract |
To disclose molecular mechanisms of cholangiocarcinogenesis and to search for novel diagnostic markers and therapeutic targets for cholangiocarcinoma, we previously analyzed gene-expression profiles of 25 intrahepatic cholangiocarcinomas (ICCs) by means of a cDNA microarray re-presenting 27,648 genes. Among the genes frequently up-regulated in the cancer cells, we focused on PSF2 (partner of SLD five 2), a component of the GINS multiprotein complex that plays a crucial role in initiation of DNA replication. Semi-quantitative RT-PCR analysis of clinical samples confirmed high levels of PSF2 expression in the cancer cells, but expression of this gene was barely detectable in normal vital organs. Transfection of ETK-1 and HuH28 cells with short-interfering RNA specific to PSF2 reduced the amount of transcript and suppressed cell growth, suggesting that PSF2 may play an important role in development of cholangio-carcinoma. The findings reported here provide new insights into human cholangiocarcinogenesis and may contribute to the development of novel therapeutic drugs for this type of liver cancer. |
| 巻・号 | 14(3) |
| ページ | 701-6 |
| 公開日 | 2005-9-1 |
| PMID | 16077978 |
| MeSH | ATP Binding Cassette Transporter, Subfamily B, Member 2 ATP Binding Cassette Transporter, Subfamily B, Member 3 ATP-Binding Cassette Transporters / genetics* Animals Bile Duct Neoplasms / genetics Bile Duct Neoplasms / pathology* Bile Ducts, Intrahepatic / metabolism Bile Ducts, Intrahepatic / pathology* Cell Line, Tumor Cell Proliferation Cells, Cultured Cholangiocarcinoma / genetics Cholangiocarcinoma / pathology* DNA-Binding Proteins / genetics Female Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Male Mice NIH 3T3 Cells Plasmids / genetics RNA Interference / physiology RNA, Small Interfering / genetics RNA, Small Interfering / metabolism Reverse Transcriptase Polymerase Chain Reaction Transfection Up-Regulation / genetics |
| IF | 3.417 |
| 引用数 | 37 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | SSP-25(RCB1293) RBE(RCB1292) ETK-1(RCB1289) |