論文 - 詳細
| RRC ID | 19202 |
|---|---|
| 著者 | Liu Z, Mai A, Sun J. |
| タイトル | Lysine acetylation regulates Bruton's tyrosine kinase in B cell activation. |
| ジャーナル | J Immunol |
| Abstract |
Bruton's tyrosine kinase (Btk) is essential for BCR signal transduction and has diverse functions in B cells. Although Btk has been extensively studied, the role of lysine acetylation in Btk regulation has not been reported. In this study, we show that BCR cross-linking induces histone lysine acetylation at the Btk promoter, correlating with marked recruitment of histone acetyltransferase E1A-associated 300-kDa protein (p300) to the locus. These effects enhance Btk promoter activity and increase the expression of Btk mRNA and protein. Consistent with these results, activated B cells display increased p300 expression and total histone acetyltransferase activity in vitro and in vivo, resulting in global histone acetylation. Interestingly, we found that BCR signaling induces Btk lysine acetylation mediated by p300. Moreover, lysine acetylation of Btk promotes its phosphorylation. Together, our results indicate a novel regulatory mechanism for Btk transcription and reveal a previously unrecognized posttranslational modification of the Btk protein and its association with phosphorylation in B cell activation. |
| 巻・号 | 184(1) |
| ページ | 244-54 |
| 公開日 | 2010-1-1 |
| DOI | 10.4049/jimmunol.0902324 |
| PII | jimmunol.0902324 |
| PMID | 19949111 |
| MeSH | Acetylation Agammaglobulinaemia Tyrosine Kinase Animals B-Lymphocytes / immunology B-Lymphocytes / metabolism* Blotting, Western Enzyme Inhibitors / pharmacology Fluorescent Antibody Technique Gene Expression Gene Expression Regulation* Histones / metabolism Lymphocyte Activation / physiology* Lysine / metabolism* Mice Mice, Inbred C57BL Oligonucleotide Array Sequence Analysis Protein Processing, Post-Translational Protein-Tyrosine Kinases / biosynthesis* Protein-Tyrosine Kinases / genetics Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / immunology* Transfection p300-CBP Transcription Factors / metabolism |
| IF | 4.886 |
| 引用数 | 10 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Lyn^(-) DT40(RCB1471) Lyn^(-)/Syk^(-) DT40(RCB1500) |