| RRC ID |
19272
|
| 著者 |
Mustonen H, Puolakkainen P, Kemppainen E, Kiviluoto T, Kivilaakso E.
|
| タイトル |
Taurocholate potentiates ethanol-induced NF-kappaB activation and inhibits caspase-3 activity in cultured rat gastric mucosal cells.
|
| ジャーナル |
Dig Dis Sci
|
| Abstract |
We have previously shown that ethanol (EtOH) induces protective NF-kappaB activation in gastric surface epithelial cells. This study investigates the defense systems in rat gastric mucosal cells (RGM-1) exposed simultaneously to EtOH and taurocholate (TC) or acetylsalicylic acid (ASA). Simultaneous exposure to ASA and EtOH increased EtOH-induced caspase-3 activity and decreased cell viability, indicating synergetic damaging action. Simultaneous exposure to TC (5 mM) with EtOH (5%) increased EtOH-induced NF-kappaB activation, opposing EtOH-induced decrease in cell membrane integrity and in cell viability as shown by decreasing RelA expression with siRNA technique. Low doses of TC decreased the EtOH (5%) induced caspase-3 activity independently from NF-kappaB pathway and inhibited EtOH-induced decrease in caspase-3 precursor protein levels, also indicating the inhibition of caspase-3 pathway. The TC (5 mM)-induced protection in EtOH exposed tissues seems to have two distinct pathways, inhibition of apoptosis and enhancement of NF-kappaB signaling.
|
| 巻・号 |
54(5)
|
| ページ |
928-36
|
| 公開日 |
2009-5-1
|
| DOI |
10.1007/s10620-008-0533-2
|
| PMID |
18989778
|
| MeSH |
Animals
Apoptosis / drug effects
Aspirin / toxicity
Caspase 3 / metabolism*
Cell Membrane / drug effects
Cell Membrane / pathology
Cell Survival / drug effects
Cells, Cultured
Dose-Response Relationship, Drug
Drug Synergism
Epithelial Cells / drug effects*
Epithelial Cells / enzymology
Epithelial Cells / pathology
Ethanol / toxicity*
Gastric Mucosa / drug effects*
Gastric Mucosa / enzymology
Gastric Mucosa / pathology
NF-kappa B / genetics
NF-kappa B / metabolism*
RNA Interference
RNA, Small Interfering / metabolism
Rats
Signal Transduction / drug effects
Taurocholic Acid / metabolism*
Transcription Factor RelA / metabolism
|
| IF |
2.751
|
| 引用数 |
6
|
|
WOS 分野
|
GASTROENTEROLOGY & HEPATOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RGM1(RCB0876) |
