RRC ID 19309
Author Lee JS, Yang EJ, Kim IS.
Title The roles of MCP-1 and protein kinase C delta activation in human eosinophilic leukemia EoL-1 cells.
Journal Cytokine
Abstract Idiopathic hypereosinophilc syndrome is a disorder associated with clonally eosinophilic proliferation. The importance of FIP1-like-1-platelet-derived growth factor receptor-alpha (FIP1L1-PDGFRA) in the pathogenesis and classification of HES has been recently reported. In this study, we investigated the contribution of monocyte chemoattractant protein-1 (MCP-1)/CCL2 to chemotactic activity and protein kinase C delta (PKC delta in the human eosinophilic leukemia cell line EoL-1. These cells express CCR2 protein among the CC chemokine receptors (CCR1-5). MCP-1 induces strong migration of EoL-1 cells and the chemotaxis signal in response to MCP-1 involves a G(i)/G(o) protein, phospholipase C (PLC), PKC delta, p38 MAPK and NF-kappaB. MCP-1 activates p38 MAPK via G(i)/G(o) protein, PLC and PKC delta cascade. MCP-1 also induces NF-kappaB translocation and the activation is inhibited by PKC delta activation. The increase in the basal expression and activity of PKC delta in EoL-1 cells, compared to normal eosinophils, inhibits apoptosis in EoL-1 cells. Anti-apoptotic mechanism of PKC delta is related to inhibition of caspase 3 and caspase 9, but not to FIP1L1-PDGFRA. PKC delta functions as an anti-apoptotic molecule, and is involved in EoL-1 cell movement stimulated by MCP-1. This study contributes to an understanding of MCP-1 in eosinophil biology and pathogenic mechanism of eosinophilic disorders.
Volume 48(3)
Pages 186-95
Published 2009-12-1
DOI 10.1016/j.cyto.2009.07.008
PII S1043-4666(09)00187-2
PMID 19664934
MeSH Apoptosis Blotting, Western Cell Line, Tumor Chemokine CCL2 / metabolism* Enzyme Activation Eosinophils / enzymology Eosinophils / metabolism Flow Cytometry Humans Hypereosinophilic Syndrome / enzymology Hypereosinophilic Syndrome / metabolism NF-kappa B / metabolism* Protein Kinase C-delta / metabolism* Reverse Transcriptase Polymerase Chain Reaction Up-Regulation
IF 2.952
Times Cited 17
Human and Animal Cells EoL-1 cell(RCB0641)