論文 - 詳細
| RRC ID | 19313 |
|---|---|
| 著者 | Munekata K, Sakamoto K. |
| タイトル | Forkhead transcription factor Foxo1 is essential for adipocyte differentiation. |
| ジャーナル | In Vitro Cell Dev Biol Anim |
| Abstract |
We analyzed the physiological role of forkhead box class O 1 (Foxo1) in adipocyte differentiation by suppressing Foxo1 mRNAwith siRNA specific for Foxo1.Mouse 3T3-L1 preadipocytes infected with an adenovirus expressing Foxo1-siRNA showed a marked decrease in lipid droplet formation when induced to differentiate into adipocytes. Adipocyte differentiation was most severely inhibited by exposing cells to Foxo1-siRNA before induction of differentiation. The incorporation of fluorescent-labeled glucose and fatty acid was significantly inhibited in cells deficient in Foxo1. RTPCR revealed that downregulation of Foxo1 decreased the expression of the transcription factors, PPAR-γ and C/EBP-α. By comparison, Foxo1-siRNA did not affect the expression of C/EBP-β or C/EBP-δ during the early period of adipocyte differentiation. These results indicate that Foxo1 plays an essential role in adipocyte differentiation, especially at the very early stage of terminal adipocyte differentiation. |
| 巻・号 | 45(10) |
| ページ | 642-51 |
| 公開日 | 2009-12-1 |
| DOI | 10.1007/s11626-009-9230-5 |
| PMID | 19585174 |
| MeSH | 3T3 Cells Adipocytes / cytology* Adipocytes / metabolism Adipogenesis / genetics Animals COS Cells Cell Differentiation / genetics* Chlorocebus aethiops Forkhead Box Protein O1 Forkhead Transcription Factors / antagonists & inhibitors Forkhead Transcription Factors / physiology* Lipid Metabolism / genetics Mice RNA Interference RNA, Messenger / metabolism |
| IF | 1.665 |
| 引用数 | 45 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | 293(RCB1637) COS-7(RCB0539) |