| RRC ID |
19314
|
| Author |
Shinkai Y, Sumi D, Toyama T, Kaji T, Kumagai Y.
|
| Title |
Role of aquaporin 9 in cellular accumulation of arsenic and its cytotoxicity in primary mouse hepatocytes.
|
| Journal |
Toxicol Appl Pharmacol
|
| Abstract |
Aquaporin (AQP) 9 is a member of the aquaglyceroporin subfamily of AQPs in the transfer of water and small solutes such as glycerol and arsenite. It is well recognized that arsenic toxicity is associated with intracellular accumulation of this metalloid. In the present study, we examined the contribution of AQP9 to the uptake of inorganic arsenite, thereby increasing arsenic-induced cytotoxicity in primary mouse hepatocytes. Pretreatment with sorbitol as a competitive inhibitor of AQP9 and siRNA-mediated knockdown of AQP9 resulted in a significant decrease of arsenite uptake in the cell and its cytotoxicity. Furthermore, overexpression of AQP9 in HEK293 cells led to the enhancement of intracellular arsenic concentration, resulting in enhanced cytotoxicity after arsenite exposure. These results suggest that AQP9 is a channel to define arsenite sensitivity in primary mouse hepatocytes.
|
| Volume |
237(2)
|
| Pages |
232-6
|
| Published |
2009-6-1
|
| DOI |
10.1016/j.taap.2009.03.014
|
| PII |
S0041-008X(09)00132-X
|
| PMID |
19341753
|
| MeSH |
Animals
Aquaporins / genetics
Aquaporins / metabolism*
Arsenic / chemistry
Arsenic / metabolism
Arsenic / toxicity
Arsenites / metabolism*
Arsenites / toxicity
Cells, Cultured
Environmental Pollutants / metabolism
Environmental Pollutants / toxicity
Enzyme Inhibitors / metabolism*
Enzyme Inhibitors / toxicity
Gene Expression Regulation
Hepatocytes / cytology
Hepatocytes / metabolism*
Humans
Male
Mice
Mice, Inbred C57BL
Molecular Structure
Sodium Compounds / metabolism*
Sodium Compounds / toxicity
Sorbitol / chemistry
Sorbitol / metabolism
Sorbitol / toxicity
|
| IF |
3.347
|
| Times Cited |
38
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
TOXICOLOGY
|
| Resource |
| Human and Animal Cells |
293(RCB1637) |
