| RRC ID |
19332
|
| Author |
Tanaka T, Kawashima H, Ohnishi K, Matsumura K, Yoshimura R, Matsuyama M, Kuratsukuri K, Nakatani T.
|
| Title |
Indirect antitumor effects of bisphosphonates on prostatic LNCaP cells co-cultured with bone cells.
|
| Journal |
Anticancer Res
|
| Abstract |
Bisphosphonates are strong inhibitors of osteoclastic bone resorption in both benign and malignant bone diseases. The nitrogen-containing bisphosphonates (N-BPs) have strong cytotoxicity via inhibition of protein prenylation in the mevalonate pathway, and also demonstrate direct cytostatic and proapoptotic effects on prostate cancer cells. We confirmed the usefulness of a co-culture system comprised of prostatic LNCaP cells, ST2 cells (mouse-derived osteoblasts) and MLC-6 cells (mouse-derived osteoclasts) in vitro. N-BPs (pamidronate and zoledronic acid) inhibited both androgen receptor transactivation and tumor cell proliferation by suppressing the activities of both osteoclasts and osteoblasts with low-dose exposure. This indirect inhibition of prostate cancer cells via bone cells could be beneficial in treating prostate cancer patients with bone metastases.
|
| Volume |
29(4)
|
| Pages |
1089-94
|
| Published |
2009-4-1
|
| PII |
29/4/1089
|
| PMID |
19414350
|
| MeSH |
Animals
Antineoplastic Agents / pharmacology
Bone Density Conservation Agents / pharmacology
Cell Proliferation / drug effects
Coculture Techniques
Diphosphonates / pharmacology*
Humans
Imidazoles / pharmacology*
Luciferases / metabolism
Male
Mice
Osteoblasts / cytology
Osteoclasts / cytology
Pamidronate
Prostatic Neoplasms / drug therapy*
Prostatic Neoplasms / metabolism
Prostatic Neoplasms / pathology
RNA, Messenger / genetics
RNA, Messenger / metabolism
Receptors, Androgen / genetics
Receptors, Androgen / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Transcriptional Activation
Tumor Cells, Cultured
Zoledronic Acid
|
| IF |
1.994
|
| Times Cited |
5
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
ST2(RCB0224)
MLC-6(RCB1536) |
