論文 - 詳細
| RRC ID | 19333 |
|---|---|
| 著者 | Uchino T, Takezawa T, Ikarashi Y. |
| タイトル | Reconstruction of three-dimensional human skin model composed of dendritic cells, keratinocytes and fibroblasts utilizing a handy scaffold of collagen vitrigel membrane. |
| ジャーナル | Toxicol In Vitro |
| Abstract |
We previously we attempted to make a three-dimensional human skin model consisting of three different cells, dendritic cells, keratinocytes and fibroblasts (KDF-Skin) to evaluate immunoreactions in human skin; however, this model had various problems; for example (1) the incubation period for the construction of this model is long (about three weeks); (2) to construct the collagen gel, high amounts of fibroblasts are needed; and (3) the horny layer of keratinocytes in this skin model is thinner than that of keratinocytes in real human skin. In order to overcome these problems, a new three-dimensional human skin model utilizing a handy scaffold of collagen vitrigel membrane (VG-KDF-Skin) was constructed. As a result, after 14 days incubation, the epidermis layer of normal human keratinocytes was thicker than the keratinocyte layer of KDF-Skin. The incubation period for VG-KDF-Skin construction was 7 days shorter than that of KDF-Skin, and the number of fibroblasts needed to seed VG-KDF-Skin was four times fewer than that of KDF-Skin. After the application of sensitizers such as DNCB, VG-KDF-Skin induced the expression of CD86 and cytokine release. These results suggest that the new three-dimensional human skin model consisting of dendritic cells, keratinocytes, fibroblasts and collagen vitrigel membrane was more useful for alternative animal testing than the KDF-Skin model. |
| 巻・号 | 23(2) |
| ページ | 333-7 |
| 公開日 | 2009-3-1 |
| DOI | 10.1016/j.tiv.2008.12.003 |
| PII | S0887-2333(08)00288-9 |
| PMID | 19121381 |
| MeSH | Animal Testing Alternatives B7-2 Antigen / metabolism Cell Culture Techniques / methods Cells, Cultured Coculture Techniques / methods Collagen Type I / chemistry Cytokines / metabolism Dendritic Cells / cytology Dendritic Cells / drug effects Dendritic Cells / metabolism* Fibroblasts / cytology Fibroblasts / drug effects Fibroblasts / metabolism* Gels Humans Irritants / toxicity Keratinocytes / cytology Keratinocytes / drug effects Keratinocytes / metabolism* Membranes, Artificial* Models, Biological Skin, Artificial* Time Factors Tissue Scaffolds* |
| IF | 2.959 |
| 引用数 | 26 |
| WOS 分野 | TOXICOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NHSF46(RCB0162) |