論文 - 詳細
| RRC ID | 19346 |
|---|---|
| 著者 | Ishii T, Uto T, Mori K, Fujikawa R. |
| タイトル | Integrin-linked kinase is involved in lactoferrin-induced anchorage-independent cell growth and survival in PC12 cells. |
| ジャーナル | Life Sci |
| Abstract |
AIMS:Bovine lactoferrin (bLf) causes anchorage-independent cell growth in PC12 cells. The present study investigated the mechanisms involved in bLf-induced anchorage-independent cell growth and survival in PC12 cells. MAIN METHODS:The number of adherent cells and suspended cells was estimated separately by using a methyl thiazol tetrazolium (MTT) assay, and the sum of both optical density (O.D.) (570 nm) values was used as a measure of the total number of cells. KEY FINDINGS:Integrin-linked kinase (ILK) plays an important role in integrin and growth factor signaling pathways. Stable transfection of PC12 cells with a dominant negative kinase-deficient mutant of ILK (DN-ILK) inhibited bLf-induced anchorage-independent cell growth. The ILK activity in the parental cells was transiently activated after addition of bLf, whereas bLf-induced activation of ILK was blocked in DN-ILK-transfected cells. bLf also activated p38 mitogen-activated protein kinase (MAPK); however, the p38 MAPK activation was inhibited by stable DN-ILK transfection. Moreover, cell viability in the suspended cells by bLf strongly decreased after treatment with SB203580, an inhibitor of p38 MAPK. SIGNIFICANCE:These results suggest that ILK is involved in bLf-induced anchorage-independent cell growth and viability via activation of p38 MAPK. |
| 巻・号 | 84(15-16) |
| ページ | 530-6 |
| 公開日 | 2009-4-10 |
| DOI | 10.1016/j.lfs.2009.01.025 |
| PII | S0024-3205(09)00051-4 |
| PMID | 19230838 |
| MeSH | Animals Cattle Cell Adhesion / drug effects Cell Culture Techniques Cell Proliferation / drug effects* Cell Survival / drug effects Lactoferrin / pharmacology* PC12 Cells Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism Protein Serine-Threonine Kinases / physiology* Rats p38 Mitogen-Activated Protein Kinases / metabolism |
| IF | 3.647 |
| 引用数 | 3 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL PHARMACOLOGY & PHARMACY |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |