論文 - 詳細
| RRC ID | 1935 |
|---|---|
| 著者 | Keller S, Nickel J, Zhang JL, Sebald W, Mueller TD. |
| タイトル | Molecular recognition of BMP-2 and BMP receptor IA. |
| ジャーナル | Nat Struct Mol Biol |
| Abstract |
Bone morphogenetic protein-2 (BMP-2) and other members of the TGF-beta superfamily regulate the development, maintenance and regeneration of tissues and organs. Binding epitopes for these extracellular signaling proteins have been defined, but hot spots specifying binding affinity and specificity have so far not been identified. In this study, mutational and structural analyses show that epitopes of BMP-2 and the BRIA receptor form a new type of protein-protein interface. The main chain atoms of Leu 51 and Asp53 of BMP-2 represent a hot spot of binding to BRIA. The BMP-2 variant L51P was deficient in type I receptor binding only, whereas its overall structure and its binding to type II receptors and modulator proteins, such as noggin, were unchanged. Thus, the L51P substitution converts BMP-2 into a receptor-inactive inhibitor of noggin. These results are relevant for other proteins of the TGF-beta superfamily and provide useful clues for structure-based drug design. |
| 巻・号 | 11(5) |
| ページ | 481-8 |
| 公開日 | 2004-5-1 |
| DOI | 10.1038/nsmb756 |
| PII | nsmb756 |
| PMID | 15064755 |
| MeSH | Animals Bone Morphogenetic Protein 2 Bone Morphogenetic Protein Receptors, Type I Bone Morphogenetic Proteins / chemistry* Bone Morphogenetic Proteins / metabolism Carrier Proteins Cell Line, Tumor Crystallography, X-Ray Hydrogen Bonding Mice Models, Molecular Protein Binding Protein Conformation Protein Serine-Threonine Kinases / chemistry* Protein Serine-Threonine Kinases / metabolism Proteins / metabolism Receptors, Growth Factor / chemistry* Receptors, Growth Factor / metabolism Transforming Growth Factor beta* |
| IF | 11.98 |
| 引用数 | 155 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 87 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 6.0 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |