Reference - Detail
|Author||Matsuzawa T, Hara F, Tohda H, Uemura H, Takegawa K.|
|Title||Promotion of glycerol utilization using ethanol and 1-propanol in Schizosaccharomyces pombe.|
|Journal||Appl. Microbiol. Biotechnol.|
The fission yeast Schizosaccharomyces pombe does not grow in media containing glycerol as a sole carbon source but uses glycerol in the presence of ethanol. Ethanol, but not glycerol, triggered upregulation of gld1+ and fbp1+ during glucose starvation even though gld1+ and fbp1+ are essential for growth on glycerol. This upregulation occurred at a very low concentration of ethanol. The transcriptional regulation of gld1+ was tested in the presence of various alcohols, and both ethanol and 1-propanol were found to induce gld1+ and to support growth in glycerol-containing media. We suggest that S. pombe has a novel ethanol and/or 1-propanol recognition mechanism that upregulates glycerol utilization during glucose starvation.
|MeSH||1-Propanol / metabolism* Carbon / metabolism Culture Media / chemistry Ethanol / metabolism* Fructose-Bisphosphatase / biosynthesis Gene Expression Gene Expression Regulation, Fungal* Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / biosynthesis Glycerol / metabolism* Schizosaccharomyces / genetics Schizosaccharomyces / growth & development Schizosaccharomyces / metabolism* Transcription, Genetic Up-Regulation|
|WOS Category||BIOTECHNOLOGY & APPLIED MICROBIOLOGY|
|Yeast||FY18624 FY18625 FY7512|