RRC ID 19379
Author Matsuzawa T, Hara F, Tohda H, Uemura H, Takegawa K.
Title Promotion of glycerol utilization using ethanol and 1-propanol in Schizosaccharomyces pombe.
Journal Appl. Microbiol. Biotechnol.
Abstract The fission yeast Schizosaccharomyces pombe does not grow in media containing glycerol as a sole carbon source but uses glycerol in the presence of ethanol. Ethanol, but not glycerol, triggered upregulation of gld1+ and fbp1+ during glucose starvation even though gld1+ and fbp1+ are essential for growth on glycerol. This upregulation occurred at a very low concentration of ethanol. The transcriptional regulation of gld1+ was tested in the presence of various alcohols, and both ethanol and 1-propanol were found to induce gld1+ and to support growth in glycerol-containing media. We suggest that S. pombe has a novel ethanol and/or 1-propanol recognition mechanism that upregulates glycerol utilization during glucose starvation.
Volume 95(2)
Pages 441-9
Published 2012-7
DOI 10.1007/s00253-012-3971-x
PMID 22370953
MeSH 1-Propanol / metabolism* Carbon / metabolism Culture Media / chemistry Ethanol / metabolism* Fructose-Bisphosphatase / biosynthesis Gene Expression Gene Expression Regulation, Fungal* Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / biosynthesis Glycerol / metabolism* Schizosaccharomyces / genetics Schizosaccharomyces / growth & development Schizosaccharomyces / metabolism* Transcription, Genetic Up-Regulation
IF 3.34
Times Cited 2
Yeast FY18624 FY18625 FY7512