論文 - 詳細
| RRC ID | 19382 |
|---|---|
| 著者 | Yano H, Yamamoto-Hino M, Awano W, Aoki-Kinoshita KF, Tsuda-Sakurai K, Okano H, Goto S. |
| タイトル | Identification of proteasome components required for apical localization of Chaoptin using functional genomics. |
| ジャーナル | J Neurogenet |
| Abstract |
Abstract: the distinct localization of membrane proteins with regard to cell polarity is crucial for the structure and function of various organs in multicellular organisms. However, the molecules and mechanisms that regulate protein localization to particular subcellular domains are still largely unknown. To identify the genes involved in regulation of protein localization, the authors performed a large-scale screen using a Drosophila RNA interference (RNAi) library, by which Drosophila genes could be knocked down in a tissue- and stage-specific manner. Drosophila photoreceptor cells have a morphologically distinct apicobasal polarity, along which Chaoptin (Chp), a glycosylphosphatidylinositol (GPI)-anchored membrane protein, and the Na (+) , K(+) -ATPase are localized to the apical and basolateral domains, respectively. By examining the subcellular localization of these proteins, the authors identified 106 genes whose knockdown resulted in mislocalization of Chp and Na(+) , K(+) -ATPase. Gene ontology analysis revealed that the knockdown of proteasome components resulted in mislocalization of Chp to the basolateral plasma membrane. These results suggest that the proteasome is involved, directly or indirectly, in selective localization of Chp to the apical plasma membrane of Drosophila photoreceptor cells. |
| 巻・号 | 26(1) |
| ページ | 53-63 |
| 公開日 | 2012-3-1 |
| DOI | 10.3109/01677063.2012.661497 |
| PMID | 22417167 |
| MeSH | Animals Cell Polarity / genetics* Databases, Genetic Drosophila / genetics Drosophila / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism* Genomics Membrane Glycoproteins / genetics Membrane Glycoproteins / metabolism* Proteasome Endopeptidase Complex / genetics Proteasome Endopeptidase Complex / metabolism* RNA Interference Subcellular Fractions / metabolism |
| IF | 1.438 |
| 引用数 | 4 |
| WOS 分野 | GENETICS & HEREDITY NEUROSCIENCES |
| リソース情報 | |
| ショウジョウバエ | 1090R-1 1109R-1 1443R-1 1763R-2 1852R-2 1973R-2 2023R-1 2221R-3 2711R-1 2848R-1 3541R-3 3758R-1 4037R-2 4582R-2 4778R-3 5044R-1 5073R-2 5358R-2 5397R-1 5820R-2 5879R-3 6159R-2 6189R-2 6226R-4 6565R-1 6586R-2 6987R-2 7382R-6 7828R-2 8144R-3 8714R-3 8768R-1 9090R-1 9364R-1 10186R-1 10202R-1 10743R-2 10960R-2 11180R-2 11427R-1 12140R-1 12623R-1 13391R-4 13995R-3 14656R-3 14932R-2 15040R-1 16742R-3 16998R-3 31082R-8 31093R-3 31241R-2 31559R-1 32389R-2 3033R-2 11190R-3 5991R-3 1884R-3 2807R-3 3314R-2 3329R-1 4904R-2 5081R-4 5670R-2 7039R-2 7283R-3 8309R-1 8542R-1 8922R-1 10230R-1 10750R-4 11981R-3 15442R-2 15506R-2 17489R-1 18495R-3 30382R-1 10701R-1 15694R-2 1404R-2 2168R-3 4550R-2 6056R-2 6615R-3 6831R-1 11687R-2 12607R-3 14334R-2 17611R-1 1228Ra-3 1291R-1 1951R-1 2958-1R-1 7038R-2 7137R-2 7556R-3 9813Ra-1 9901Rb-1 10445Rb-1 9324R-1 13638R-1 2666R-2 4759R-3 5731R-3 6750R-3 7619R-4 |