RRC ID 19632
著者 Tanaka H, Murakami Y, Ishii I, Nakata S.
タイトル Involvement of a forkhead transcription factor, FOXO1A, in UV-induced changes of collagen metabolism.
ジャーナル J Investig Dermatol Symp Proc
Abstract Transcription factors belonging to the forkhead box gene, group O (FOXOs) family have been found to be crucial in downstream suppression of life-shortening effects of the insulin/insulin-like growth factor-1 (IGF-1) signaling pathway, which accelerates aging by suppressing FOXOs. Thus, FOXOs could hold the key for counteracting aging. Although FOXOs may play a critical role in aging, the effects of FOXOs on UV-induced changes of collagen metabolism by dermal fibroblasts are unknown. In this study, UV-induced changes in FOXO1a expression and the roles of FOXO1a in the regulation of collagen synthesis and matrix metalloproteinase (MMPs) expression in human dermal fibroblasts were investigated. In UVA- or UVB-irradiated fibroblasts, the expression of FOXO1A mRNA decreased significantly. The expression of type I collagen (COLIAI) also decreased. On the other hand, MMP-1 and MMP-2 mRNA levels increased. FOXO1A-small interfering RNA transfection induced the downregulation of FOXO1A expression, it also induced a decrease in COLIAI expression, and it increased MMP-1 and MMP-2 expression. These changes are similar to those observed in UV-irradiated fibroblasts. Furthermore, FOXO1a-peptide induced opposite changes in COLIAI, MMP-1, and MMP-2 expression. Therefore, FOXO1a is involved in the UV-induced changes of type I collagen and MMPs expression.Journal of Investigative Dermatology Symposium Proceedings (2009) 14, 60-62; doi:10.1038/jidsymp.2009.2.
巻・号 14(1)
ページ 60-2
公開日 2009-8-1
DOI 10.1038/jidsymp.2009.2
PII S1087-0024(15)30513-X
PMID 19675556
MeSH Base Sequence Cell Line Collagen / metabolism* Collagen Type I / genetics Collagen Type I, alpha 1 Chain DNA Primers / genetics Fibroblasts / metabolism Fibroblasts / radiation effects Forkhead Box Protein O1 Forkhead Transcription Factors / antagonists & inhibitors Forkhead Transcription Factors / genetics* Forkhead Transcription Factors / metabolism* Gene Expression / radiation effects Humans Matrix Metalloproteinase 1 / genetics Matrix Metalloproteinase 2 / genetics RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / genetics Recombinant Proteins / genetics Recombinant Proteins / metabolism Skin / metabolism* Skin / radiation effects* Ultraviolet Rays / adverse effects*
引用数 12
WOS 分野 DERMATOLOGY
リソース情報
ヒト・動物細胞 NB1RGB(RCB0222)