| RRC ID |
19676
|
| 著者 |
Sasakura Y, Kanda M, Ikeda T, Horie T, Kawai N, Ogura Y, Yoshida R, Hozumi A, Satoh N, Fujiwara S.
|
| タイトル |
Retinoic acid-driven Hox1 is required in the epidermis for forming the otic/atrial placodes during ascidian metamorphosis.
|
| ジャーナル |
Development
|
| Abstract |
Retinoic acid (RA)-mediated expression of the homeobox gene Hox1 is a hallmark of the chordate central nervous system (CNS). It has been suggested that the RA-Hox1 network also functions in the epidermal ectoderm of chordates. Here, we show that in the urochordate ascidian Ciona intestinalis, RA-Hox1 in the epidermal ectoderm is necessary for formation of the atrial siphon placode (ASP), a structure homologous to the vertebrate otic placode. Loss of Hox1 function resulted in loss of the ASP, which could be rescued by expressing Hox1 in the epidermis. As previous studies showed that RA directly upregulates Hox1 in the epidermis of Ciona larvae, we also examined the role of RA in ASP formation. We showed that abolishment of RA resulted in loss of the ASP, which could be rescued by forced expression of Hox1 in the epidermis. Our results suggest that RA-Hox1 in the epidermal ectoderm played a key role in the acquisition of the otic placode during chordate evolution.
|
| 巻・号 |
139(12)
|
| ページ |
2156-60
|
| 公開日 |
2012-6-1
|
| DOI |
10.1242/dev.080234
|
| PII |
dev.080234
|
| PMID |
22573621
|
| MeSH |
Animals
Ciona intestinalis / drug effects
Ciona intestinalis / growth & development*
Enhancer Elements, Genetic / genetics
Epidermis / drug effects
Epidermis / growth & development*
Epidermis / metabolism
Gene Expression Regulation, Developmental / drug effects
Gills / drug effects
Gills / growth & development
Gills / metabolism
Green Fluorescent Proteins / metabolism
Heart Atria / anatomy & histology*
Heart Atria / drug effects
Heart Atria / growth & development*
Heart Atria / metabolism
Homeodomain Proteins / genetics
Homeodomain Proteins / metabolism*
Metamorphosis, Biological / drug effects*
Metamorphosis, Biological / genetics
Muscle Development / drug effects
Muscles / drug effects
Mutation / genetics
Tretinoin / pharmacology*
|
| IF |
5.611
|
| 引用数 |
35
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
pBSCiHox1 (RDB08929) |
| カタユウレイボヤ・(ニッポンウミシダ) |
no gill slit(EJ[MiTSAdTPOG]124)
Tg[MiCiTnIGCiprmG]2
Tg[MiCiEpiIG]4
Tg[MiCiEpiIG]3
Tg[MiCiTnIG]2
pBSCiHox1
pSPCiTTF1eCFPCiHox1
pSPCib2TBeCFPCiHox1
pSPCiAKReCFPCiHox1
pSPCiCesAeCFPCiHox1
pSPeCFPCiHox1
pSPeCFP-ter
pRN3CiHox1
Wild C. int |
