RRC ID 19676
Author Sasakura Y, Kanda M, Ikeda T, Horie T, Kawai N, Ogura Y, Yoshida R, Hozumi A, Satoh N, Fujiwara S.
Title Retinoic acid-driven Hox1 is required in the epidermis for forming the otic/atrial placodes during ascidian metamorphosis.
Journal Development
Abstract Retinoic acid (RA)-mediated expression of the homeobox gene Hox1 is a hallmark of the chordate central nervous system (CNS). It has been suggested that the RA-Hox1 network also functions in the epidermal ectoderm of chordates. Here, we show that in the urochordate ascidian Ciona intestinalis, RA-Hox1 in the epidermal ectoderm is necessary for formation of the atrial siphon placode (ASP), a structure homologous to the vertebrate otic placode. Loss of Hox1 function resulted in loss of the ASP, which could be rescued by expressing Hox1 in the epidermis. As previous studies showed that RA directly upregulates Hox1 in the epidermis of Ciona larvae, we also examined the role of RA in ASP formation. We showed that abolishment of RA resulted in loss of the ASP, which could be rescued by forced expression of Hox1 in the epidermis. Our results suggest that RA-Hox1 in the epidermal ectoderm played a key role in the acquisition of the otic placode during chordate evolution.
Volume 139(12)
Pages 2156-60
Published 2012-6
DOI 10.1242/dev.080234
PII dev.080234
PMID 22573621
MeSH Animals Ciona intestinalis / drug effects Ciona intestinalis / growth & development* Enhancer Elements, Genetic / genetics Epidermis / drug effects Epidermis / growth & development* Epidermis / metabolism Gene Expression Regulation, Developmental / drug effects Gills / drug effects Gills / growth & development Gills / metabolism Green Fluorescent Proteins / metabolism Heart Atria / anatomy & histology* Heart Atria / drug effects Heart Atria / growth & development* Heart Atria / metabolism Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* Metamorphosis, Biological / drug effects* Metamorphosis, Biological / genetics Muscle Development / drug effects Muscles / drug effects Mutation / genetics Tretinoin / pharmacology*
IF 5.763
Times Cited 24
WOS Category DEVELOPMENTAL BIOLOGY
Resource
DNA material pBSCiHox1 (RDB08929)
C.intestinalis / (O.japonicus) no gill slit(EJ[MiTSAdTPOG]124) Tg[MiCiTnIGCiprmG]2 Tg[MiCiEpiIG]4 Tg[MiCiEpiIG]3 Tg[MiCiTnIG]2 pBSCiHox1 pSPCiTTF1eCFPCiHox1 pSPCib2TBeCFPCiHox1 pSPCiAKReCFPCiHox1 pSPCiCesAeCFPCiHox1 pSPeCFPCiHox1 pSPeCFP-ter pRN3CiHox1 Wild C. int