| RRC ID |
1978
|
| Author |
Lievremont JP, Numaga T, Vazquez G, Lemonnier L, Hara Y, Mori E, Trebak M, Moss SE, Bird GS, Mori Y, Putney JW Jr.
|
| Title |
The role of canonical transient receptor potential 7 in B-cell receptor-activated channels.
|
| Journal |
J Biol Chem
|
| Abstract |
Phospholipase C signaling stimulates Ca2+ entry across the plasma membrane through multiple mechanisms. Ca2+ store depletion stimulates store-operated Ca2+-selective channels, or alternatively, other phospholipase C-dependent events activate Ca2+-permeable non-selective cation channels. Transient receptor potential 7 (TRPC7) is a non-selective cation channel that can be activated by both mechanisms when ectopically expressed, but the regulation of native TRPC7 channels is not known. We knocked out TRPC7 in DT40 B-cells, which expresses both forms of Ca2+ entry. No difference in the store-operated current I(crac) was detected between TRPC7-/- and wild-type cells. Wild-type cells demonstrated nonstore-operated cation entry and currents in response to activation of the B-cell receptor or protease-activated receptor 2, intracellular dialysis with GTPgammaS, or application of the synthetic diacylglycerol oleyl-acetyl-glycerol. These responses were absent in TRPC7-/- cells but could be restored by transfection with human TRPC7. In conclusion, in B-lymphocytes, TRPC7 appeared to participate in the formation of ion channels that could be activated by phospholipase C-linked receptors. This represents the first demonstration of a physiological function for endogenous TRPC7 channels.
|
| Volume |
280(42)
|
| Pages |
35346-51
|
| Published |
2005-10-21
|
| DOI |
10.1074/jbc.M507606200
|
| PII |
S0021-9258(19)48207-0
|
| PMID |
16123040
|
| MeSH |
Animals
B-Lymphocytes / metabolism
Barium / metabolism
Blotting, Northern
Calcium / metabolism*
Calcium Channels / chemistry
Cations
Cell Line
Chickens
Diglycerides / chemistry
Electrophysiology
Glycerol / chemistry
Guanosine 5'-O-(3-Thiotriphosphate) / metabolism
Humans
Ion Channels / chemistry
Ions
Models, Genetic
Receptors, Antigen, B-Cell / metabolism*
Signal Transduction
TRPC Cation Channels / metabolism
TRPC Cation Channels / physiology*
Thapsigargin / pharmacology
Time Factors
Transfection
Transgenes
Type C Phospholipases / metabolism*
|
| IF |
4.238
|
| Times Cited |
41
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
DT40(RCB1464) |
