RRC ID 2012
Author Numazawa S, Shindo S, Maruyama K, Chibana F, Kawahara Y, Ashino T, Tanaka S, Yoshida T.
Title Impaired nuclear translocation of CAR in hepatic preneoplastic lesions: association with an attenuated CYP2B induction by phenobarbital.
Journal FEBS Lett
Abstract Phenobarbital (PB) induction of CYP2B, a representative target gene of constitutive androstane receptor (CAR), has been observed to be attenuated in preneoplastic lesions of rat liver; however, molecular basis for this attenuation is poorly understood. In this report, we provide evidence indicating that the CAR expressed in the hepatic preneoplastic lesions of rats and mice was resistant to nuclear translocation and transactivation of the PB-responsive enhancer module upon PB treatment. These observations suggest that the attenuation of the induction of CYP2B by PB in hepatic preneoplastic lesions is evidently a consequence of impaired nuclear translocation of CAR.
Volume 579(17)
Pages 3560-4
Published 2005-7-4
DOI 10.1016/j.febslet.2005.05.028
PII S0014-5793(05)00636-8
PMID 15953603
MeSH Active Transport, Cell Nucleus / drug effects Animals Cell Nucleus / chemistry Cell Nucleus / metabolism* Cytochrome P-450 CYP2B1 / genetics Cytochrome P-450 CYP2B1 / metabolism* Down-Regulation Liver / drug effects* Liver / pathology Liver Neoplasms / metabolism* Male Mice Phenobarbital / pharmacology* Precancerous Conditions / metabolism* Rats Receptors, Cytoplasmic and Nuclear / analysis Receptors, Cytoplasmic and Nuclear / genetics Receptors, Cytoplasmic and Nuclear / metabolism* Transcription Factors / analysis Transcription Factors / genetics Transcription Factors / metabolism* Transcriptional Activation / drug effects
IF 2.675
Times Cited 9
DNA material P450 2B1 (CYP2B1) cDNA (RDB01245)