RRC ID 2024
Author Nishioka K, Fujimori Y, Hashimoto-Tamaoki T, Kai S, Qiu H, Kobayashi N, Tanaka N, Westerman KA, Leboulch P, Hara H.
Title Immortalization of bone marrow-derived human mesenchymal stem cells by removable simian virus 40T antigen gene: analysis of the ability to support expansion of cord blood hematopoietic progenitor cells.
Journal Int J Oncol
Abstract Human marrow-derived mesenchymal stem cells (MSC), which have the potential to differentiate into mesenchymal tissues, such as bone, cartilage, adipose and bone marrow stroma, were transduced with a retroviral vector carrying the simian virus 40 large T antigen, hygromycin-resistant gene and herpes simplex virus thymidine kinase gene, that can be excised by Cre/loxP site-specific recombination. This resulted in establishment of an MSC cell line, HMSC-1, which retained original surface characteristics and differentiation potential, and exhibited a higher proliferative capacity than parental cells. HMSC-1 expressed mRNAs of BMP-4, Jagged-1, and SCF that are known to promote hematopoiesis. Human CB CD34+ hematopoietic progenitor cells (HPC) cultured on a layer of HMSC-1 cells showed high expansion of CD34+CD38- immature HPC, capable of reconstituting human hematopoiesis in non-obese diabetic/severe combined immunodeficient disease (NOD/SCID) mice. This cell line may be of value for developing strategies for ex vivo expansion of human HPC.
Volume 23(4)
Pages 925-32
Published 2003-10-1
PMID 12963970
MeSH ADP-ribosyl Cyclase / biosynthesis ADP-ribosyl Cyclase 1 Animals Antigens, CD / biosynthesis Antigens, CD34 / biosynthesis Antigens, Polyomavirus Transforming / genetics* Blotting, Western Bone Marrow Cells / cytology* Cell Culture Techniques / methods Cell Differentiation Cinnamates / pharmacology Coculture Techniques Colony-Forming Units Assay Fetal Blood / cytology* Flow Cytometry Hematopoietic Stem Cells / cytology* Humans Hygromycin B / analogs & derivatives* Hygromycin B / pharmacology Membrane Glycoproteins Mesoderm / cytology* Mice Mice, SCID Models, Genetic Phenotype RNA, Messenger / metabolism Recombination, Genetic Retroviridae / genetics Reverse Transcriptase Polymerase Chain Reaction Stem Cells / cytology* Time Factors
IF 3.899
Times Cited 20
DNA material AxCANCre (RDB01748)