Reference - Detail
| RRC ID | 2027 |
|---|---|
| Author | Yazawa M, Setoguchi A, Hong SH, Uyama R, Nakagawa T, Kanaya N, Nishimura R, Sasaki N, Masuda K, Ohno K, Tsujimoto H. |
| Title | Effect of an adenoviral vector that expresses the canine p53 gene on cell growth of canine osteosarcoma and mammary adenocarcinoma cell lines. |
| Journal | Am J Vet Res |
| Abstract |
OBJECTIVE:To generate an adenoviral vector that expressed the canine p53 gene and investigate its growth-inhibiting effect on canine osteosarcoma and mammary adenocarcinoma cell lines. SAMPLE POPULATION:2 canine osteosarcoma cell lines (HOS, OOS) and 3 canine mammary adenocarcinoma cell lines (CHMp, CIPm, and CNMm). PROCEDURE:An adenoviral vector that expressed the canine p53 gene (AxCA-cp53) was generated. p53 gene expression was examined by use of reverse transcription (RT)-polymerase chain reaction (PCR) assay and immunohistochemistry. Susceptibility of cell lines to the adenoviral vector was determined by infection with an adenoviral vector that expresses beta-galactosidase (AxCA-LacZ) and 3-indolyl-beta-D-galactopyranoside staining. Growth inhibitory effects were examined by monitoring the numbers of cells after infection with mock (PBS) solution, AxCA-LacZ, or AxCA-cp53. The DNA contents per cell were measured by flow cytometry analysis. Apoptotic DNA fragmentation was detected by use of a terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay. RESULTS:AxCA-cp53-derived p53 gene mRNA and P53 protein were detected by RT-PCR analysis and immunohistochemistry, respectively. Multiplicity of infection at which 50% of cells had positive 3-indolyl-beta-D-galactopyranoside staining results ranged from 10 to 50. AxCA-cp53 induced growth inhibition in a dose-dependent manner. Arrest of the G1-phase population and apoptotic DNA fragmentation were observed in cells infected with AxCA-cp53. CONCLUSIONS AND CLINICAL RELEVANCE:AxCA-cp53 inhibits cell growth via induction of cell cycle arrest and apoptosis in canine osteosarcoma and mammary adenocarcinoma cell lines that lack a functional p53 gene. AxCA-cp53 may be useful to target the p53 gene in the treatment of dogs with tumors. |
| Volume | 64(7) |
| Pages | 880-8 |
| Published | 2003-7-1 |
| DOI | 10.2460/ajvr.2003.64.880 |
| PMID | 12856773 |
| MeSH | Adenocarcinoma / genetics Adenocarcinoma / pathology* Adenocarcinoma / veterinary Adenoviridae / genetics* Animals Apoptosis Cell Cycle Cell Division Dogs Female Gene Expression Genes, p53 / genetics* Mammary Neoplasms, Animal / genetics Mammary Neoplasms, Animal / pathology* Osteosarcoma / genetics Osteosarcoma / pathology* Osteosarcoma / veterinary Tumor Cells, Cultured Tumor Suppressor Protein p53 / genetics Tumor Suppressor Protein p53 / metabolism |
| IF | 0.811 |
| Times Cited | 6 |
| WOS Category | VETERINARY SCIENCES |
| Resource | |
| DNA material | AxCALacZ (RDB1745) |