RRC ID 20279
著者 Sekimoto H, Fukumoto R, Dohmae N, Takio K, Fujii T, Kamiya Y.
タイトル Molecular cloning of a novel sex pheromone responsible for the release of a different sex pheromone in Closterium peracerosum-strigosum-littorale complex.
ジャーナル Plant Cell Physiol
Abstract A sex pheromone, protoplast-release-inducing protein (PR-IP) inducer, of the Closterium peracerosum-strigosum-littorale complex is known to induce the release of PR-IP, from mating-type plus (mt+) cells during sexual reproduction. The purified PR-IP inducer was treated with trypsin to obtain internal peptides for determination of partial amino acid sequences. Using these sequences, oligonucleotides were synthesized and used as primers for the combined reverse transcription-PCR. A 296 bp cDNA fragment was amplified, permitting the cloning of corresponding full length cDNA (CpPI; Closterium peracerosum-strigosum-littorale complex PR-IP inducer). The deduced amino acid sequence of CpPI encodes a protein of 212 amino acid residues of M(r) 23,071 whereas portion of the peptide secreted is predicted to have 142 amino acid residues of M(r) 15,717 and shows no significant similarity with known proteins. The predicted protein has three possible consensus sequences for asparagine-linked glycosylation site. The CpPI gene was expressed when mating-type minus (mt-) cells were incubated at a low cell density in the light. Nitrogen deprivation from the medium enhances expression of the CpPI gene. An analysis by genomic Southern hybridization revealed that the cDNA probe hybridized to several DNA fragments obtained from both the genome of mt- and mt+ cells. However, in mt- cells, transcripts for the PR-IP inducer could not be detected by Northern hybridization.
巻・号 39(11)
ページ 1169-75
公開日 1998-11-1
DOI 10.1093/oxfordjournals.pcp.a029317
PMID 9891415
MeSH Amino Acid Sequence Base Sequence Cloning, Molecular Conjugation, Genetic Molecular Sequence Data Peptide Fragments / chemistry Plant Physiological Phenomena* Plants / genetics* Recombinant Proteins / biosynthesis Recombinant Proteins / chemistry Recombinant Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Sex Attractants / chemistry Sex Attractants / genetics* Sex Attractants / physiology*
IF 4.062
引用数 23
WOS 分野 PLANT SCIENCES CELL BIOLOGY
リソース情報
藻類 NIES-67 NIES-68