Reference - Detail
| RRC ID | 2030 |
|---|---|
| Author | Morita N, Yasumori T, Nakayama K. |
| Title | Human MDR1 polymorphism: G2677T/A and C3435T have no effect on MDR1 transport activities. |
| Journal | Biochem Pharmacol |
| Abstract |
The two most frequently observed single nucleotide polymorphisms (SNPs) of the human multidrug resistance 1 (MDR1) gene are 2677G/T/A (893Ala/Ser/Thr) and 3435C/T (no amino acid substitution). In this study, six forms of MDR1 cDNAs with the SNPs were expressed in LLC-PK1 cells and their transport activities were determined. Nearly identical amounts of the recombinant MDR1 proteins were expressed in the established cell lines using the Flp recombinase, which integrates a gene of interest at a specific genomic location. Four structurally diverse compounds: verapamil, digoxin, vinblastine and cyclosporin A, were examined for transcellular transport activities and intracellular accumulation. No significant differences were observed between cells expressing five polymorphic types of the MDR1 cDNAs (2677G/3435T, 2677A/3435C, 2677A/3435T, 2677T/3435C, 2677T/3435T) and cells expressing the wild-type (2677G/3435C). These results suggested that the two frequently observed MDR1 SNPs had no effect on the transport activities of MDR1 proteins expressed in LLC-PK1 cells in vitro, and other genetic or environmental factors might control the expression of MDR1 and the in vivo activity of MDR1. |
| Volume | 65(11) |
| Pages | 1843-52 |
| Published | 2003-6-1 |
| DOI | 10.1016/s0006-2952(03)00178-3 |
| PII | S0006295203001783 |
| PMID | 12781336 |
| MeSH | ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism* Animals Biological Transport Cells, Cultured Genome, Human Genotype* Humans Point Mutation Polymorphism, Single Nucleotide Recombinant Proteins / metabolism |
| IF | 4.96 |
| Times Cited | 101 |
| WOS Category | PHARMACOLOGY & PHARMACY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | Human MDR1 cDNA (RDB01372) |