RRC ID 20372
著者 Yubuki N, Inagaki Y, Nakayama T, Inouye I.
タイトル Ultrastructure and ribosomal RNA phylogeny of the free-living heterotrophic flagellate Dysnectes brevis n. gen., n. sp., a new member of the Fornicata.
ジャーナル J Eukaryot Microbiol
Abstract Dysnectes brevis n. gen., n. sp., a free-living heterotrophic flagellate that grows under microaerophilic conditions possesses two flagella. The posterior one lies in a ventral feeding groove, suggesting that this flagellate is an excavate. Our detailed electron microscopic observations revealed that D. brevis possesses all the key ultrastructural characters considered typical of Excavata. Among the 10 excavate groups previously recognized, D. brevis displays an evolutionary affinity to members of the Fornicata (i.e. Carpediemonas, retortamonads, and diplomonads). Firstly, a strong D. brevis-Fornicata affinity was recovered in the phylogenetic analyses of small subunit ribosomal RNA (SSU rRNA) sequences, albeit the internal branching pattern of the D. brevis+Fornicata clade was not resolved with confidence. Corresponding to the SSU rRNA phylogeny, D. brevis and the Fornicata shared the following components of the flagellar apparatus: the arched B fiber bridging the right root; a posterior basal body; and a left root. Combining both morphological and molecular phylogenetic analyses, D. brevis is classified as a new free-living excavate in the Fornicata incertae sedis.
巻・号 54(2)
ページ 191-200
公開日 2007-1-1
DOI 10.1111/j.1550-7408.2007.00252.x
PII JEU252
PMID 17403160
MeSH Animals DNA, Protozoan / chemistry DNA, Protozoan / genetics Eukaryota / classification Eukaryota / genetics* Eukaryota / ultrastructure* Evolution, Molecular Microscopy, Electron, Transmission Molecular Sequence Data Phylogeny* RNA, Ribosomal, 18S / genetics Sequence Analysis, DNA
IF 2.143
引用数 25
WOS 分野 MICROBIOLOGY
リソース情報
藻類 NIES-1843