Reference - Detail
| RRC ID | 20386 |
|---|---|
| Author | Makiuchi T, Nara T, Annoura T, Hashimoto T, Aoki T. |
| Title | Occurrence of multiple, independent gene fusion events for the fifth and sixth enzymes of pyrimidine biosynthesis in different eukaryotic groups. |
| Journal | Gene |
| Abstract |
The genes encoding orotate phosphoribosyltransferase (OPRT) and orotidine-5'-monophosphate decarboxylase (OMPDC), the fifth and sixth enzymes in the de novo pyrimidine biosynthetic pathway, are fused as OPRT-OMPDC in most eukaryotic groups. On the other hand, the inversely linked OMPDC-OPRT fusion is present in trypanosomatids, belonging to kinetoplastids together with bodonids in a supergroup, Euglenozoa. Here, we show the presence of OMPDC-OPRT in the bodonid, Bodo caudatus, while OPRT-OMPDC in Euglena gracilis, another euglenozoan species belonging to euglenoids. These results suggest that the OMPDC-OPRT fusion event occurred in a common ancestor of kinetoplastids. Genome sequence database searches further revealed the presence of OMPDC-OPRT in stramenopiles and cyanobacteria. Phylogenetic reconstruction of OPRT and OMPDC rejected statistically the monophyly of the OPRT domains of stramenopile and kinetoplastid OMPDC-OPRT, demonstrating that these gene fusions do not share a common evolutionary origin, despite the identical gene order. Thus, the OMPDC-OPRT fusion is likely to have emerged independently in these eukaryotic groups. Phylogenetic analyses also suggested that cyanobacterial OMPDC-OPRT arose via lateral transfer. We conclude that gene fusion events occur more frequently than previously thought and that lateral gene transfer has made a marked contribution to establishment of the rearranged structure of OPRT and OMPDC genes in eukaryotes. |
| Volume | 394(1-2) |
| Pages | 78-86 |
| Published | 2007-6-1 |
| DOI | 10.1016/j.gene.2007.02.009 |
| PII | S0378-1119(07)00089-3 |
| PMID | 17383832 |
| MeSH | Animals Cloning, Molecular Cyanobacteria / genetics Cyanobacteria / metabolism DNA, Algal / genetics DNA, Complementary / genetics DNA, Protozoan / genetics Euglena gracilis / genetics* Euglena gracilis / metabolism* Evolution, Molecular Gene Fusion* Gene Transfer, Horizontal Genes, Protozoan Kinetoplastida / genetics* Kinetoplastida / metabolism* Models, Genetic Molecular Sequence Data Phylogeny Pyrimidines / biosynthesis* Species Specificity |
| IF | 2.984 |
| Times Cited | 16 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Algae | NIES-48 |