RRC ID 20869
Author Ogawa Y, Kimura S, Wada M, Kuga S.
Title Crystal analysis and high-resolution imaging of microfibrillar α-chitin from Phaeocystis.
Journal J. Struct. Biol.
Abstract The ultrastructure of α-chitin microfibril produced by marine alga Phaeocystis was investigated by FT-IR spectroscopy, X-ray diffraction and electron microscopy. The average size of the microfibril was 17.1±1.8 μm in length and 39.8±8.8 nm in width. The FT-IR spectrum shows typical α-chitin pattern, and each band was sharper than crustacean chitin's, indicating higher crystallinity of the Phaeocystis chitin. The X-ray diffraction gave crystallite size more than twice of crustacean tendon's. The fiber diffraction pattern is consistent with previous studies with two-chains orthorhombic unit cell (Minke and Blackwell, 1978; Sikorski et al., 2009), and refined unit cell dimensions are a=4.742 Å, b=18.871 Å, c=10.338Å. High-resolution electron microscopy of ultrathin sections gave the cross-sectional shape of microfibril as hexagon. The lattice images of (020) plane (d=0.94 nm) were frequently observed extending the entire cross-section of microfibril, indicating its single crystalline nature. These results allowed construction of a molecular packing model for α-chitin crystal.
Volume 171(1)
Pages 111-6
Published 2010-7
DOI 10.1016/j.jsb.2010.03.010
PII S1047-8477(10)00087-0
PMID 20350601
MeSH Chitin / chemistry* Crystallization Haptophyta / chemistry Microfibrils / chemistry* Microscopy, Electron Models, Molecular Spectroscopy, Fourier Transform Infrared X-Ray Diffraction
IF 3.433
Times Cited 19
WOS Category BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Algae NIES-1396