Reference - Detail
| RRC ID | 20869 |
|---|---|
| Author | Ogawa Y, Kimura S, Wada M, Kuga S. |
| Title | Crystal analysis and high-resolution imaging of microfibrillar α-chitin from Phaeocystis. |
| Journal | J Struct Biol |
| Abstract |
The ultrastructure of α-chitin microfibril produced by marine alga Phaeocystis was investigated by FT-IR spectroscopy, X-ray diffraction and electron microscopy. The average size of the microfibril was 17.1±1.8 μm in length and 39.8±8.8 nm in width. The FT-IR spectrum shows typical α-chitin pattern, and each band was sharper than crustacean chitin's, indicating higher crystallinity of the Phaeocystis chitin. The X-ray diffraction gave crystallite size more than twice of crustacean tendon's. The fiber diffraction pattern is consistent with previous studies with two-chains orthorhombic unit cell (Minke and Blackwell, 1978; Sikorski et al., 2009), and refined unit cell dimensions are a=4.742 Å, b=18.871 Å, c=10.338Å. High-resolution electron microscopy of ultrathin sections gave the cross-sectional shape of microfibril as hexagon. The lattice images of (020) plane (d=0.94 nm) were frequently observed extending the entire cross-section of microfibril, indicating its single crystalline nature. These results allowed construction of a molecular packing model for α-chitin crystal. |
| Volume | 171(1) |
| Pages | 111-6 |
| Published | 2010-7-1 |
| DOI | 10.1016/j.jsb.2010.03.010 |
| PII | S1047-8477(10)00087-0 |
| PMID | 20350601 |
| MeSH | Chitin / chemistry* Crystallization Haptophyta / chemistry Microfibrils / chemistry* Microscopy, Electron Models, Molecular Spectroscopy, Fourier Transform Infrared X-Ray Diffraction |
| IF | 3.071 |
| Times Cited | 36 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Algae | NIES-1396 |