| RRC ID |
20869
|
| 著者 |
Ogawa Y, Kimura S, Wada M, Kuga S.
|
| タイトル |
Crystal analysis and high-resolution imaging of microfibrillar α-chitin from Phaeocystis.
|
| ジャーナル |
J Struct Biol
|
| Abstract |
The ultrastructure of α-chitin microfibril produced by marine alga Phaeocystis was investigated by FT-IR spectroscopy, X-ray diffraction and electron microscopy. The average size of the microfibril was 17.1±1.8 μm in length and 39.8±8.8 nm in width. The FT-IR spectrum shows typical α-chitin pattern, and each band was sharper than crustacean chitin's, indicating higher crystallinity of the Phaeocystis chitin. The X-ray diffraction gave crystallite size more than twice of crustacean tendon's. The fiber diffraction pattern is consistent with previous studies with two-chains orthorhombic unit cell (Minke and Blackwell, 1978; Sikorski et al., 2009), and refined unit cell dimensions are a=4.742 Å, b=18.871 Å, c=10.338Å. High-resolution electron microscopy of ultrathin sections gave the cross-sectional shape of microfibril as hexagon. The lattice images of (020) plane (d=0.94 nm) were frequently observed extending the entire cross-section of microfibril, indicating its single crystalline nature. These results allowed construction of a molecular packing model for α-chitin crystal.
|
| 巻・号 |
171(1)
|
| ページ |
111-6
|
| 公開日 |
2010-7-1
|
| DOI |
10.1016/j.jsb.2010.03.010
|
| PII |
S1047-8477(10)00087-0
|
| PMID |
20350601
|
| MeSH |
Chitin / chemistry*
Crystallization
Haptophyta / chemistry
Microfibrils / chemistry*
Microscopy, Electron
Models, Molecular
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction
|
| IF |
3.071
|
| 引用数 |
36
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 藻類 |
NIES-1396 |
