論文 - 詳細
| RRC ID | 20935 |
|---|---|
| 著者 | Myosho T, Otake H, Masuyama H, Matsuda M, Kuroki Y, Fujiyama A, Naruse K, Hamaguchi S, Sakaizumi M. |
| タイトル | Tracing the emergence of a novel sex-determining gene in medaka, Oryzias luzonensis. |
| ジャーナル | Genetics |
| Abstract |
Three sex-determining (SD) genes, SRY (mammals), Dmy (medaka), and DM-W (Xenopus laevis), have been identified to date in vertebrates. However, how and why a new sex-determining gene appears remains unknown, as do the switching mechanisms of the master sex-determining gene. Here, we used positional cloning to search for the sex-determining gene in Oryzias luzonensis and found that GsdfY (gonadal soma derived growth factor on the Y chromosome) has replaced Dmy as the master sex-determining gene in this species. We found that GsdfY showed high expression specifically in males during sex differentiation. Furthermore, the presence of a genomic fragment that included GsdfY converts XX individuals into fertile XX males. Luciferase assays demonstrated that the upstream sequence of GsdfY contributes to the male-specific high expression. Gsdf is downstream of Dmy in the sex-determining cascade of O. latipes, suggesting that emergence of the Dmy-independent Gsdf allele led to the appearance of this novel sex-determining gene in O. luzonensis. |
| 巻・号 | 191(1) |
| ページ | 163-70 |
| 公開日 | 2012-5-1 |
| DOI | 10.1534/genetics.111.137497 |
| PII | genetics.111.137497 |
| PMID | 22367037 |
| PMC | PMC3338257 |
| MeSH | Amino Acid Sequence Animals Computational Biology Female Fertility / genetics Fish Proteins / chemistry Fish Proteins / genetics Fish Proteins / metabolism Gene Expression Regulation Male Molecular Sequence Data Mutation Oryzias / genetics* Oryzias / physiology Sex Characteristics Sex Chromosomes / genetics Sex Determination Processes / genetics* |
| IF | 4.015 |
| 引用数 | 224 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | O. luzonensis (RS270) d-rR Hd-rR-Tg-GsdfY (TG1318) |