RRC ID |
20935
|
著者 |
Myosho T, Otake H, Masuyama H, Matsuda M, Kuroki Y, Fujiyama A, Naruse K, Hamaguchi S, Sakaizumi M.
|
タイトル |
Tracing the emergence of a novel sex-determining gene in medaka, Oryzias luzonensis.
|
ジャーナル |
Genetics
|
Abstract |
Three sex-determining (SD) genes, SRY (mammals), Dmy (medaka), and DM-W (Xenopus laevis), have been identified to date in vertebrates. However, how and why a new sex-determining gene appears remains unknown, as do the switching mechanisms of the master sex-determining gene. Here, we used positional cloning to search for the sex-determining gene in Oryzias luzonensis and found that GsdfY (gonadal soma derived growth factor on the Y chromosome) has replaced Dmy as the master sex-determining gene in this species. We found that GsdfY showed high expression specifically in males during sex differentiation. Furthermore, the presence of a genomic fragment that included GsdfY converts XX individuals into fertile XX males. Luciferase assays demonstrated that the upstream sequence of GsdfY contributes to the male-specific high expression. Gsdf is downstream of Dmy in the sex-determining cascade of O. latipes, suggesting that emergence of the Dmy-independent Gsdf allele led to the appearance of this novel sex-determining gene in O. luzonensis.
|
巻・号 |
191(1)
|
ページ |
163-70
|
公開日 |
2012-5-1
|
DOI |
10.1534/genetics.111.137497
|
PII |
genetics.111.137497
|
PMID |
22367037
|
PMC |
PMC3338257
|
MeSH |
Amino Acid Sequence
Animals
Computational Biology
Female
Fertility / genetics
Fish Proteins / chemistry
Fish Proteins / genetics
Fish Proteins / metabolism
Gene Expression Regulation
Male
Molecular Sequence Data
Mutation
Oryzias / genetics*
Oryzias / physiology
Sex Characteristics
Sex Chromosomes / genetics
Sex Determination Processes / genetics*
|
IF |
4.015
|
引用数 |
224
|
WOS 分野
|
GENETICS & HEREDITY
|
リソース情報 |
メダカ |
O. luzonensis (RS270)
d-rR
Hd-rR-Tg-GsdfY (TG1318) |