RRC ID 20935
Author Myosho T, Otake H, Masuyama H, Matsuda M, Kuroki Y, Fujiyama A, Naruse K, Hamaguchi S, Sakaizumi M.
Title Tracing the emergence of a novel sex-determining gene in medaka, Oryzias luzonensis.
Journal Genetics
Abstract Three sex-determining (SD) genes, SRY (mammals), Dmy (medaka), and DM-W (Xenopus laevis), have been identified to date in vertebrates. However, how and why a new sex-determining gene appears remains unknown, as do the switching mechanisms of the master sex-determining gene. Here, we used positional cloning to search for the sex-determining gene in Oryzias luzonensis and found that GsdfY (gonadal soma derived growth factor on the Y chromosome) has replaced Dmy as the master sex-determining gene in this species. We found that GsdfY showed high expression specifically in males during sex differentiation. Furthermore, the presence of a genomic fragment that included GsdfY converts XX individuals into fertile XX males. Luciferase assays demonstrated that the upstream sequence of GsdfY contributes to the male-specific high expression. Gsdf is downstream of Dmy in the sex-determining cascade of O. latipes, suggesting that emergence of the Dmy-independent Gsdf allele led to the appearance of this novel sex-determining gene in O. luzonensis.
Volume 191(1)
Pages 163-70
Published 2012-5-1
DOI 10.1534/genetics.111.137497
PII genetics.111.137497
PMID 22367037
PMC PMC3338257
MeSH Amino Acid Sequence Animals Computational Biology Female Fertility / genetics Fish Proteins / chemistry Fish Proteins / genetics Fish Proteins / metabolism Gene Expression Regulation Male Molecular Sequence Data Mutation Oryzias / genetics* Oryzias / physiology Sex Characteristics Sex Chromosomes / genetics Sex Determination Processes / genetics*
IF 3.564
Times Cited 224
Medaka O. luzonensis (RS270) d-rR Hd-rR-Tg-GsdfY (TG1318)