RRC ID 20955
Author Froschauer A, Khatun MM, Sprott D, Franz A, Rieger C, Pfennig F, Gutzeit HO.
Title oct4-EGFP reporter gene expression marks the stem cells in embryonic development and in adult gonads of transgenic medaka.
Journal Mol. Reprod. Dev.
Abstract Maintenance of pluripotency in stem cells is tightly regulated among vertebrates. One of the key genes in this process is oct4, also referred to as pou5f1 in mammals and pou2 in teleosts. Pou5f1 evolved by duplication of pou2 early in the tetrapod lineage, but only monotremes and marsupials retained both genes. Either pou2 or pou5f1 was lost from the genomes of the other tetrapods that have been analyzed to date. Consequently, these two homologous genes are often designated oct4 in functional studies. In most vertebrates oct4 is expressed in pluripotent cells of the early embryo until the blastula stage, and later persist in germline stem cells until adulthood. The isolation and analysis of stem cells from embryo or adult individuals is hampered by the need for reliable markers that can identify and define the cell populations. Here, we report the faithful expression of EGFP under the control of endogenous pou2/oct4 promoters in transgenic medaka (Oryzias latipes). In vivo imaging in oct4-EGFP transgenic medaka reveals the temporal and spatial expression of pou2 in embryos and adults alike. We describe the temporal and spatial patterns of endogenous pou2 and oct4-EGFP expression in medaka with respect to germline and adult stem cells, and discuss applications of oct4-EGFP transgenic medaka in reproductive and stem cell biology.
Volume 80(1)
Pages 48-58
Published 2013-1
DOI 10.1002/mrd.22135
PMID 23139203
MeSH Animals Animals, Genetically Modified Brain / metabolism Cloning, Molecular Embryonic Development / genetics* Embryonic Development / physiology Female Flow Cytometry Genes, Reporter Gonads / metabolism* Green Fluorescent Proteins / biosynthesis Green Fluorescent Proteins / genetics* Green Fluorescent Proteins / metabolism Histocytochemistry Male Microscopy, Confocal Octamer Transcription Factor-3 / genetics* Octamer Transcription Factor-3 / metabolism Oryzias Recombinant Fusion Proteins / biosynthesis Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Stem Cells / cytology Stem Cells / metabolism Stem Cells / physiology*
IF 3.113
Times Cited 9
WOS Category REPRODUCTIVE BIOLOGY DEVELOPMENTAL BIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Medaka FLFII (MT109) STII i-3 gu (MT58) Medaka BAC clone