論文 - 詳細
| RRC ID | 20962 |
|---|---|
| 著者 | Kimura K, Kuwayama H, Amagai A, Maeda Y. |
| タイトル | Developmental significance of cyanide-resistant respiration under stressed conditions: experiments in Dictyostelium cells. |
| ジャーナル | Dev Growth Differ |
| Abstract |
We have previously reported that benzohydroxamic acid (BHAM), a potent inhibitor of cyanide (CN)-resistant respiration mediated by alternative oxidase (AOX), induces formation of unique cell masses (i.e., stalk-like cells with a large vacuole and thick cell wall) in starved Dictyostelium cells. Unexpectedly, however, aox-null cells prepared by homologous recombination exhibited normal development under normal culture conditions on agar, indicating that BHAM-induced stalk formation is not solely attributable to inhibition of CN-resistant respiration. This also suggests that a series of pharmacological approaches in the field of life science has serious limitations. Under stress (e.g., in submerged culture), starved aox-null cells exhibited slightly delayed aggregation compared with parental Ax-2 cells; most cells remained as loose aggregates even after prolonged incubation. Also, the developmental defects of aox-null cells became more marked upon incubation for 30 min just after starvation in the presence of ≥ 1.75 mmol/L H(2)O(2). This seems to indicate that CN-resistant respiration could mitigate cellular damage through reactive oxygen species (ROS), because AOX has a potential role in reduction of ROS production. Starved aox-null cells did not develop in the presence of 5 mmol/L KCN (which completely inhibited the conventional cytochrome-mediated respiration) and remained as non-aggregated single cells on agar even after prolonged incubation. Somewhat surprisingly, however, parental Ax-2 cells were found to develop normally, forming fruiting bodies even in the presence of 10 mmol/L KCN. Taken together, these results suggest that CN-resistant respiration might compensate for the production of adenosine tri-phosphate via oxidative phosphorylation. |
| 巻・号 | 52(7) |
| ページ | 645-56 |
| 公開日 | 2010-9-1 |
| DOI | 10.1111/j.1440-169X.2010.01200.x |
| PII | DGD1200 |
| PMID | 20887565 |
| MeSH | Adenosine Triphosphate / biosynthesis Cell Respiration / drug effects Dictyostelium / cytology Dictyostelium / drug effects Dictyostelium / growth & development* Dictyostelium / metabolism* Mitochondrial Proteins Oxidative Stress / drug effects* Oxidoreductases / metabolism Phosphorylation Plant Proteins Potassium Cyanide / pharmacology* Reactive Oxygen Species / metabolism |
| IF | 1.723 |
| 引用数 | 10 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 細胞性粘菌 | G21281 |