RRC ID 21099
Author Du H, Yang L, Wu J, Xiao L, Wang X, Jiang L.
Title Simultaneous removal of phosphorus and nitrogen in a sequencing batch biofilm reactor with transgenic bacteria expressing polyphosphate kinase.
Journal Appl. Microbiol. Biotechnol.
Abstract To improve phosphorus removal from wastewater, we constructed a high-phosphate-accumulating microorganism, KTPPK, using Pseudomonas putida KT2440 as a host. The expression plasmid was constructed by inserting and expressing polyphosphate kinase gene (ppk) from Microcystis aeruginosa NIES-843 into broad-host-range plasmid, pBBR1MCS-2. KTPPK was then added to a sequencing batch biofilm reactor (SBBFR) using lava as a biological carrier. The results showed that SBBFR with KTPPK not only efficiently removed COD, NH(3)-N, and NO(3)(-)-N but also had a high removal capacity for PO(4)(3-)-P, resulting in a low phosphorus concentration remaining in the outflow of the SBBFR. The biofilm increased by 30-53% on the lava in the SBBFR that contained KTPPK after 11 days when compared with the reactor that contained P. putida KT2440. Real-time quantitative polymerase chain reaction confirmed that the copy of ppk was maintained at about 3.5 × 10(10) copies per μL general DNA in the biofilm after 20 days. Thus, the transgenic bacteria KTPPK could maintain a high density and promote phosphorus removal in the SBBFR. In summary, this study indicates that the use of SBBFR with transgenic bacteria has the potential to remove phosphorus and nitrogen from wastewater.
Volume 96(1)
Pages 265-72
Published 2012-10
DOI 10.1007/s00253-011-3839-5
PMID 22218771
MeSH Biofilms / growth & development* Bioreactors / microbiology Gene Expression Profiling Genomic Instability Metabolic Engineering* Microcystis / enzymology Microcystis / genetics Nitrogen / metabolism* Organisms, Genetically Modified / genetics Organisms, Genetically Modified / metabolism* Organisms, Genetically Modified / physiology Phosphorus / metabolism* Phosphotransferases (Phosphate Group Acceptor) / genetics Phosphotransferases (Phosphate Group Acceptor) / metabolism* Plasmids Pseudomonas putida / genetics Pseudomonas putida / metabolism* Pseudomonas putida / physiology Real-Time Polymerase Chain Reaction Recombinant Proteins / genetics Recombinant Proteins / metabolism
IF 3.67
Times Cited 5
Algae NIES-843