論文 - 詳細
| RRC ID | 21414 |
|---|---|
| 著者 | Hou J, Liu Y, Lu Z, Liu X, Liu J. |
| タイトル | Biochemical characterization of RNase HII from Aeropyrum pernix. |
| ジャーナル | Acta Biochim Biophys Sin (Shanghai) |
| Abstract |
Aeropyrum pernix contains one homolog of ribonuclease H (RNase H), A. pernix RNase HII (Ape-RNase HII). Activity characterization showed that Ape-RNase HII exhibited the highest activity in the presence of 5 mM Mn(2+), 1 mM Co(2+), or 10 mM Mg(2+), respectively; however, its cleavage efficiencies at different cleavage sites for Mn(2+) and Mg(2+) were different. Ape-RNase HII cleaved 12-bp RNA/DNA substrates at multiple sites and the optimum pH value was 11.0. Moreover, 16-bp DNA-r4-DNA/DNA and 13-bp DNA-r1-DNA/DNA chimeric substrates were cleaved at DNA-RNA junction. Ape-RNase HII was thermostable and the stabilization was enhanced with increased salt concentration. This work is believed to be the first in vitro functional study of Ape-RNase HII and the results should contribute to the analysis of RNase H of other archaeal species. |
| 巻・号 | 44(4) |
| ページ | 339-46 |
| 公開日 | 2012-4-1 |
| DOI | 10.1093/abbs/gms011 |
| PII | gms011 |
| PMID | 22366566 |
| MeSH | Aeropyrum / enzymology* Aeropyrum / genetics Amino Acid Sequence Archaeal Proteins / genetics Archaeal Proteins / metabolism* Binding Sites / genetics Biocatalysis / drug effects Cobalt / pharmacology DNA, Archaeal / genetics DNA, Archaeal / metabolism Electrophoresis, Polyacrylamide Gel Enzyme Stability Hydrogen-Ion Concentration Kinetics Manganese / pharmacology Molecular Sequence Data RNA, Archaeal / genetics RNA, Archaeal / metabolism Recombinant Proteins / metabolism Ribonuclease H / genetics Ribonuclease H / metabolism* Sequence Homology, Amino Acid Substrate Specificity Temperature |
| IF | 2.836 |
| 引用数 | 6 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 9820 |