RRC ID |
21414
|
著者 |
Hou J, Liu Y, Lu Z, Liu X, Liu J.
|
タイトル |
Biochemical characterization of RNase HII from Aeropyrum pernix.
|
ジャーナル |
Acta Biochim Biophys Sin (Shanghai)
|
Abstract |
Aeropyrum pernix contains one homolog of ribonuclease H (RNase H), A. pernix RNase HII (Ape-RNase HII). Activity characterization showed that Ape-RNase HII exhibited the highest activity in the presence of 5 mM Mn(2+), 1 mM Co(2+), or 10 mM Mg(2+), respectively; however, its cleavage efficiencies at different cleavage sites for Mn(2+) and Mg(2+) were different. Ape-RNase HII cleaved 12-bp RNA/DNA substrates at multiple sites and the optimum pH value was 11.0. Moreover, 16-bp DNA-r4-DNA/DNA and 13-bp DNA-r1-DNA/DNA chimeric substrates were cleaved at DNA-RNA junction. Ape-RNase HII was thermostable and the stabilization was enhanced with increased salt concentration. This work is believed to be the first in vitro functional study of Ape-RNase HII and the results should contribute to the analysis of RNase H of other archaeal species.
|
巻・号 |
44(4)
|
ページ |
339-46
|
公開日 |
2012-4-1
|
DOI |
10.1093/abbs/gms011
|
PII |
gms011
|
PMID |
22366566
|
MeSH |
Aeropyrum / enzymology*
Aeropyrum / genetics
Amino Acid Sequence
Archaeal Proteins / genetics
Archaeal Proteins / metabolism*
Binding Sites / genetics
Biocatalysis / drug effects
Cobalt / pharmacology
DNA, Archaeal / genetics
DNA, Archaeal / metabolism
Electrophoresis, Polyacrylamide Gel
Enzyme Stability
Hydrogen-Ion Concentration
Kinetics
Manganese / pharmacology
Molecular Sequence Data
RNA, Archaeal / genetics
RNA, Archaeal / metabolism
Recombinant Proteins / metabolism
Ribonuclease H / genetics
Ribonuclease H / metabolism*
Sequence Homology, Amino Acid
Substrate Specificity
Temperature
|
IF |
2.836
|
引用数 |
6
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
一般微生物 |
JCM 9820 |