論文 - 詳細
| RRC ID | 21504 |
|---|---|
| 著者 | Sun L, Liu O, Desai J, Karbassi F, Sylvain MA, Shi A, Zhou Z, Rocheleau CE, Grant BD. |
| タイトル | CED-10/Rac1 regulates endocytic recycling through the RAB-5 GAP TBC-2. |
| ジャーナル | PLoS Genet |
| Abstract |
Rac1 is a founding member of the Rho-GTPase family and a key regulator of membrane remodeling. In the context of apoptotic cell corpse engulfment, CED-10/Rac1 acts with its bipartite guanine nucleotide exchange factor, CED-5/Dock180-CED-12/ELMO, in an evolutionarily conserved pathway to promote phagocytosis. Here we show that in the context of the Caenorhabditis elegans intestinal epithelium CED-10/Rac1, CED-5/Dock180, and CED-12/ELMO promote basolateral recycling. Furthermore, we show that CED-10 binds to the RAB-5 GTPase activating protein TBC-2, that CED-10 contributes to recruitment of TBC-2 to endosomes, and that recycling cargo is trapped in recycling endosomes in ced-12, ced-10, and tbc-2 mutants. Expression of GTPase defective RAB-5(Q78L) also traps recycling cargo. Our results indicate that down-regulation of early endosome regulator RAB-5/Rab5 by a CED-5, CED-12, CED-10, TBC-2 cascade is an important step in the transport of cargo through the basolateral recycling endosome for delivery to the plasma membrane. |
| 巻・号 | 8(7) |
| ページ | e1002785 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pgen.1002785 |
| PII | PGENETICS-D-12-00595 |
| PMID | 22807685 |
| PMC | PMC3395619 |
| MeSH | Animals Apoptosis Regulatory Proteins Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans* / physiology Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Carrier Proteins / metabolism Cell Membrane / metabolism Cytoskeletal Proteins / metabolism Endocytosis* / genetics Endocytosis* / physiology Endosomes / metabolism Endosomes / physiology GTPase-Activating Proteins* / genetics GTPase-Activating Proteins* / metabolism Gene Expression Regulation Intestinal Mucosa / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism Mutation Phagocytosis / genetics Protein Transport Signal Transduction Vesicular Transport Proteins* / genetics Vesicular Transport Proteins* / metabolism rac GTP-Binding Proteins* / genetics rac GTP-Binding Proteins* / metabolism |
| IF | 5.175 |
| 引用数 | 12 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| 線虫 | tm2241 |