RRC ID 2153
Author Kim YH, Ishikawa D, Ha HP, Sugiyama M, Kaneko Y, Harashima S.
Title Chromosome XII context is important for rDNA function in yeast.
Journal Nucleic Acids Res.
Abstract The rDNA cluster in Saccharomyces cerevisiae is located 450 kb from the left end and 610 kb from the right end of chromosome XII and consists of approximately 150 tandemly repeated copies of a 9.1 kb rDNA unit. To explore the biological significance of this specific chromosomal context, chromosome XII was split at both sides of the rDNA cluster and strains harboring deleted variants of chromosome XII consisting of 450 kb, 1500 kb (rDNA cluster only) and 610 kb were created. In the strain harboring the 1500 kb variant of chromosome XII consisting solely of rDNA, the size of the rDNA cluster was found to decrease as a result of a decrease in rDNA copy number. The frequency of silencing of URA3 inserted within the rDNA locus was found to be greater than in a wild-type strain. The localization and morphology of the nucleolus was also affected such that a single and occasionally (6-12% frequency) two foci for Nop1p and a rounded nucleolus were observed, whereas a typical crescent-shaped nucleolar structure was seen in the wild-type strain. Notably, strains harboring the 450 kb chromosome XII variant and/or the 1500 kb variant consisting solely of rDNA had shorter life spans than wild type and also accumulated extrachromosomal rDNA circles. These observations suggest that the context of chromosome XII plays an important role in maintaining a constant rDNA copy number and in physiological processes related to rDNA function in S.cerevisiae.
Volume 34(10)
Pages 2914-24
Published 2006
DOI 10.1093/nar/gkl293
PII 34/10/2914
PMID 16738130
PMC PMC1474064
MeSH Cell Nucleolus / ultrastructure Chromosome Deletion Chromosomes, Artificial, Yeast Chromosomes, Fungal* DNA, Ribosomal / genetics* DNA, Ribosomal / physiology* Gene Dosage Gene Silencing Genes, rRNA* Multigene Family* Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / growth & development Saccharomyces cerevisiae / ultrastructure
IF 11.561
Times Cited 19
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Yeast BY5209(FY833) BY5210(FY834) BY5675(W303-1B) BY611(S288C) BYP3121(p3121) BYP3008(p3008) BYP3009(p3009) BYP3010(p3010)