論文 - 詳細
| RRC ID | 21551 |
|---|---|
| 著者 | International Barley Genome Sequencing Consortium, Mayer KF, Waugh R, Brown JW, Schulman A, Langridge P, Platzer M, Fincher GB, Muehlbauer GJ, Sato K, Close TJ, Wise RP, Stein N. |
| タイトル | A physical, genetic and functional sequence assembly of the barley genome. |
| ジャーナル | Nature |
| Abstract |
Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement. |
| 巻・号 | 491(7426) |
| ページ | 711-6 |
| 公開日 | 2012-11-29 |
| DOI | 10.1038/nature11543 |
| PII | nature11543 |
| PMID | 23075845 |
| MeSH | Alternative Splicing / genetics Codon, Nonsense / genetics Crops, Agricultural / genetics Evolution, Molecular Gene Expression Regulation, Plant Genes, Plant / genetics Genome, Plant / genetics* Genomics Hordeum / classification Hordeum / genetics* Molecular Sequence Annotation Physical Chromosome Mapping Polymorphism, Single Nucleotide / genetics Repetitive Sequences, Nucleic Acid / genetics Sequence Analysis, DNA* Transcriptome / genetics |
| IF | 42.779 |
| 引用数 | 887 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 162 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| オオムギ | Full length cDNA Haruna Nijo |