RRC ID 21551
著者 International Barley Genome Sequencing Consortium, Mayer KF, Waugh R, Brown JW, Schulman A, Langridge P, Platzer M, Fincher GB, Muehlbauer GJ, Sato K, Close TJ, Wise RP, Stein N.
タイトル A physical, genetic and functional sequence assembly of the barley genome.
ジャーナル Nature
Abstract Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.
巻・号 491(7426)
ページ 711-6
公開日 2012-11-29
DOI 10.1038/nature11543
PII nature11543
PMID 23075845
MeSH Alternative Splicing / genetics Codon, Nonsense / genetics Crops, Agricultural / genetics Evolution, Molecular Gene Expression Regulation, Plant Genes, Plant / genetics Genome, Plant / genetics* Genomics Hordeum / classification Hordeum / genetics* Molecular Sequence Annotation Physical Chromosome Mapping Polymorphism, Single Nucleotide / genetics Repetitive Sequences, Nucleic Acid / genetics Sequence Analysis, DNA* Transcriptome / genetics
IF 42.779
引用数 887
リソース情報
オオムギ Full length cDNA Haruna Nijo