| RRC ID |
2175
|
| 著者 |
Tanaka N, Fujita Y, Suzuki S, Morishita M, Giga-Hama Y, Shimoda C, Takegawa K.
|
| タイトル |
Characterization of O-mannosyltransferase family in Schizosaccharomyces pombe.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Protein O-glycosylation is an essential protein modification in eukaryotic cells. In Saccharomyces cerevisiae, O-mannosylation is initiated in the lumen of the endoplasmic reticulum by O-mannosyltransferase gene products (Pmt1p-7p). A search of the Schizosaccharomyces pombe genome database revealed a total of three O-glycoside mannosyltransferase homologs (ogm1+, ogm2+, and ogm4+), closely related to Saccharomyces cerevisiae PMT1, PMT2, and PMT4. Although individual ogm genes were not found to be essential, ogm1Delta and ogm4Delta mutants exhibited aberrant morphology and failed to agglutinate during mating. The phenotypes of the ogm4Delta mutant were not complemented by overexpression of ogm1+ or ogm2+, suggesting that each of the Ogm proteins does not have overlapping functions. Heterologous expression of a chitinase from S. cerevisiae in the ogm mutants revealed that O-glycosylation of chitinase had decreased in ogm1Delta cells. A GFP-tagged Fus1p from S. cerevisiae was specifically not glycosylated and accumulated in the Golgi in ogm4Delta cells. These results indicate that O-glycosylation initiated by Ogm proteins plays crucial physiological roles and can serve as a sorting determinant for protein transport of membrane glycoproteins in S. pombe.
|
| 巻・号 |
330(3)
|
| ページ |
813-20
|
| 公開日 |
2005-5-13
|
| DOI |
10.1016/j.bbrc.2005.03.033
|
| PII |
S0006-291X(05)00522-X
|
| PMID |
15809069
|
| MeSH |
Animals
Cell Division / genetics
Chitinases / metabolism
Glycosylation
Golgi Apparatus / metabolism
Humans
Mannose / metabolism
Mannosyltransferases / classification*
Mannosyltransferases / genetics
Mannosyltransferases / metabolism*
Membrane Proteins
Mutation / genetics
Phenotype
Phylogeny
Protein Transport
Saccharomyces cerevisiae / enzymology
Saccharomyces cerevisiae Proteins / metabolism
Schizosaccharomyces / enzymology*
Schizosaccharomyces / genetics
|
| IF |
2.985
|
| 引用数 |
20
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 酵母 |
KJ100-7B |
