論文 - 詳細
| RRC ID | 21805 |
|---|---|
| 著者 | Yazawa H, Holic R, Kumagai H, Uemura H. |
| タイトル | Toxicity of ricinoleic acid production in fission yeast Schizosaccharomyces pombe is suppressed by the overexpression of plg7, a phospholipase A2 of a platelet-activating factor (PAF) family homolog. |
| ジャーナル | Appl Microbiol Biotechnol |
| Abstract |
In an effort to produce ricinoleic acid (RA), an important natural raw material with great values as a petrochemical replacement, in Schizosaccharomyces pombe, we introduced Claviceps purpurea oleate Δ12-hydroxylase gene (CpFAH12) to S. pombe, putting it under the control of an inducible nmt1 promoter. However, RA was toxic to S. pombe and the cells expressing CpFAH12 grew poorly at the normal growth temperature 30 °C. To address its toxic mechanism in S. pombe, we screened for a S. pombe cDNA library and identified plg7, which encodes a phospholipase A2, as a suppressor that restored the growth defect without affecting the RA production. A lacZ fusion experiment showed that the expression of plg7 was inducible by RA. Thin layer chromatographic analysis confirmed a reduction in RA moiety in phospholipids and a concomitant increase in free RA in the plg7 overexpressed strain. Since RA is synthesized at the sn-2 position of phosphatidylcholine by Fah12p, and phospholipase A2 hydrolyzes the sn-2 acyl bond of phospholipids, we speculate that plg7 is a stress-responsive gene, and removal of RA moieties from phospholipids, major components of lipid bilayer membrane, by Plg7p would be its suppression mechanism. |
| 巻・号 | 97(18) |
| ページ | 8193-203 |
| 公開日 | 2013-9-1 |
| DOI | 10.1007/s00253-013-4987-6 |
| PMID | 23700240 |
| MeSH | Down-Regulation Metabolic Engineering Phospholipases A2 / genetics* Phospholipases A2 / metabolism Ricinoleic Acids / metabolism* Ricinoleic Acids / toxicity* Schizosaccharomyces / drug effects Schizosaccharomyces / enzymology* Schizosaccharomyces / genetics Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism |
| IF | 3.53 |
| 引用数 | 10 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FYG173_l08 FYG100_p12 FYG83_c12 FYG168_a16 FYG105_o07 FYG177_i21 FYG90_d01 FYG62_j22 FYG81_f23 FYG107_c22 FYG133_l20 FYG84_e18 FYG20_j07 FYG108_o11 FYG104_i21 FYG72_c19 FYG50_f08 FYG117_f20 FYG111_n08 FYG70_m20 FYG164_l04 FYG118_j12 FYG166_l09 FYG159_o20 FYG9_d04 FYG146_p21 FYG27_c12 FYG123_j08 FYG143_c15 FYG180_m03 |