| RRC ID |
21805
|
| 著者 |
Yazawa H, Holic R, Kumagai H, Uemura H.
|
| タイトル |
Toxicity of ricinoleic acid production in fission yeast Schizosaccharomyces pombe is suppressed by the overexpression of plg7, a phospholipase A2 of a platelet-activating factor (PAF) family homolog.
|
| ジャーナル |
Appl Microbiol Biotechnol
|
| Abstract |
In an effort to produce ricinoleic acid (RA), an important natural raw material with great values as a petrochemical replacement, in Schizosaccharomyces pombe, we introduced Claviceps purpurea oleate Δ12-hydroxylase gene (CpFAH12) to S. pombe, putting it under the control of an inducible nmt1 promoter. However, RA was toxic to S. pombe and the cells expressing CpFAH12 grew poorly at the normal growth temperature 30 °C. To address its toxic mechanism in S. pombe, we screened for a S. pombe cDNA library and identified plg7, which encodes a phospholipase A2, as a suppressor that restored the growth defect without affecting the RA production. A lacZ fusion experiment showed that the expression of plg7 was inducible by RA. Thin layer chromatographic analysis confirmed a reduction in RA moiety in phospholipids and a concomitant increase in free RA in the plg7 overexpressed strain. Since RA is synthesized at the sn-2 position of phosphatidylcholine by Fah12p, and phospholipase A2 hydrolyzes the sn-2 acyl bond of phospholipids, we speculate that plg7 is a stress-responsive gene, and removal of RA moieties from phospholipids, major components of lipid bilayer membrane, by Plg7p would be its suppression mechanism.
|
| 巻・号 |
97(18)
|
| ページ |
8193-203
|
| 公開日 |
2013-9-1
|
| DOI |
10.1007/s00253-013-4987-6
|
| PMID |
23700240
|
| MeSH |
Down-Regulation
Metabolic Engineering
Phospholipases A2 / genetics*
Phospholipases A2 / metabolism
Ricinoleic Acids / metabolism*
Ricinoleic Acids / toxicity*
Schizosaccharomyces / drug effects
Schizosaccharomyces / enzymology*
Schizosaccharomyces / genetics
Schizosaccharomyces / metabolism
Schizosaccharomyces pombe Proteins / genetics*
Schizosaccharomyces pombe Proteins / metabolism
|
| IF |
3.53
|
| 引用数 |
10
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 酵母 |
FYG173_l08
FYG100_p12
FYG83_c12
FYG168_a16
FYG105_o07
FYG177_i21
FYG90_d01
FYG62_j22
FYG81_f23
FYG107_c22
FYG133_l20
FYG84_e18
FYG20_j07
FYG108_o11
FYG104_i21
FYG72_c19
FYG50_f08
FYG117_f20
FYG111_n08
FYG70_m20
FYG164_l04
FYG118_j12
FYG166_l09
FYG159_o20
FYG9_d04
FYG146_p21
FYG27_c12
FYG123_j08
FYG143_c15
FYG180_m03 |
