| RRC ID |
21864
|
| Author |
Baek SH, Cho HW, Kwon YC, Lee JH, Kim MJ, Lee H, Choe KM.
|
| Title |
Requirement for Pak3 in Rac1-induced organization of actin and myosin during Drosophila larval wound healing.
|
| Journal |
FEBS Lett
|
| Abstract |
Rho-family small GTPases regulate epithelial cell sheet migration by organizing actin and myosin during wound healing. Here, we report that Pak3, but not Pak1, is a downstream target protein for Rac1 in wound closure of the Drosophila larval epidermis. Pak3-deficient larvae failed to close a wound hole and this defect was not rescued by Pak1 expression, indicating differential functions of the two proteins. Pak3 localized to the wound margin, which selectively required Rac1. Pak3-deficient larvae showed severe defects in actin-myosin organization at the wound margin and in submarginal cells, which was reminiscent of the phenotypes of Rac1-deficient larvae. These results suggest that Pak3 specifically mediates Rac1 signaling in organizing actin and myosin during Drosophila epidermal wound healing.
|
| Volume |
586(6)
|
| Pages |
772-7
|
| Published |
2012-3-23
|
| DOI |
10.1016/j.febslet.2012.01.061
|
| PII |
S0014-5793(12)00110-X
|
| PMID |
22449966
|
| MeSH |
Actins / metabolism*
Animals
Drosophila Proteins / metabolism*
Drosophila melanogaster / physiology*
Epidermis / pathology
Epidermis / physiology
Larva / anatomy & histology
Larva / physiology*
Myosins / metabolism*
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Wound Healing / physiology
p21-Activated Kinases / genetics
p21-Activated Kinases / metabolism*
rac1 GTP-Binding Protein / genetics
rac1 GTP-Binding Protein / metabolism*
|
| IF |
3.057
|
| Times Cited |
14
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Drosophila |
14895R-1
2248R-2
9774R-2
18255R-3 |
