Reference - Detail
| RRC ID | 21895 |
|---|---|
| Author | Kain P, Badsha F, Hussain SM, Nair A, Hasan G, Rodrigues V. |
| Title | Mutants in phospholipid signaling attenuate the behavioral response of adult Drosophila to trehalose. |
| Journal | Chem Senses |
| Abstract |
In Drosophila melanogaster, gustatory receptor genes (Grs) encode putative G-protein-coupled receptors (GPCRs) that are expressed in gustatory receptor neurons (GRNs). One of the Gr genes, Gr5a, encodes a receptor for trehalose that is expressed in a subset of GRNs. Although a role for the G protein, Gsα, has been shown in Gr5a-expressing taste neurons, there is the residual responses to trehalose in Gsα mutants which could suggest additional transduction mechanisms. Expression and genetic analysis of the heterotrimeric G-protein subunit, Gq, shown here suggest involvement of this Gα subunit in trehalose perception in Drosophila. A green fluorescent protein reporter of Gq expression is detected in gustatory neurons in the labellum, tarsal segments, and wing margins. Animals heterozygous for dgq mutations and RNA interference-mediated knockdown of dgq showed reduced responses to trehalose in the proboscis extension reflex assay and feeding behavior assay. These defects were rescued by targeted expression of the wild-type dgqα transgene in the GRNs. These data together with observations from other mutants in phospholipid signaling provide insights into the mechanisms of taste transduction in Drosophila. |
| Volume | 35(8) |
| Pages | 663-73 |
| Published | 2010-10-1 |
| DOI | 10.1093/chemse/bjq055 |
| PII | bjq055 |
| PMID | 20543015 |
| MeSH | Animals Behavior, Animal* Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / drug effects Drosophila melanogaster / genetics Drosophila melanogaster / physiology* Female GTP-Binding Protein alpha Subunits, Gq-G11 / genetics* GTP-Binding Protein alpha Subunits, Gq-G11 / metabolism Heterozygote Male Mutation RNA Interference Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Signal Transduction TRPC Cation Channels / genetics TRPC Cation Channels / metabolism Taste / physiology Trehalose / pharmacology* |
| IF | 2.261 |
| Times Cited | 15 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY PHYSIOLOGY BEHAVIORAL SCIENCES NEUROSCIENCES |
| Altmetric score |
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| Total number of mentions | 0 |
| Resource | |
| Drosophila | 1063R-1 1063R-2 |