論文 - 詳細
| RRC ID | 21905 |
|---|---|
| 著者 | Kametaka S, Kametaka A, Yonekura S, Haruta M, Takenoshita S, Goto S, Waguri S. |
| タイトル | AP-1 clathrin adaptor and CG8538/Aftiphilin are involved in Notch signaling during eye development in Drosophila melanogaster. |
| ジャーナル | J Cell Sci |
| Abstract |
Clathrin adaptor protein complex-1 (AP-1) and its accessory proteins play a role in the sorting of integral membrane proteins at the trans-Golgi network and endosomes. Their physiological functions in complex organisms, however, are not fully understood. In this study, we found that CG8538p, an uncharacterized Drosophila protein, shares significant structural and functional characteristics with Aftiphilin, a mammalian AP-1 accessory protein. The Drosophila Aftiphilin was shown to interact directly with the ear domain of γ-adaptin of Drosophila AP-1, but not with the GAE domain of Drosophila GGA. In S2 cells, Drosophila Aftiphilin and AP-1 formed a complex and colocalized at the Golgi compartment. Moreover, tissue-specific depletion of AP-1 or Aftiphilin in the developing eyes resulted in a disordered alignment of photoreceptor neurons in larval stage and roughened eyes with aberrant ommatidia in adult flies. Furthermore, AP-1-depleted photoreceptor neurons showed an intracellular accumulation of a Notch regulator, Scabrous, and downregulation of Notch by promoting its degradation in the lysosomes. These results suggest that AP-1 and Aftiphilin are cooperatively involved in the intracellular trafficking of Notch during eye development in Drosophila. |
| 巻・号 | 125(Pt 3) |
| ページ | 634-48 |
| 公開日 | 2012-2-1 |
| DOI | 10.1242/jcs.090167 |
| PII | 125/3/634 |
| PMID | 22389401 |
| MeSH | Adaptor Protein Complex gamma Subunits / chemistry Adaptor Protein Complex gamma Subunits / genetics Adaptor Protein Complex gamma Subunits / metabolism Amino Acid Sequence Animals Carrier Proteins / genetics Carrier Proteins / metabolism Cell Compartmentation Cell Line Drosophila Proteins / chemistry Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development* Drosophila melanogaster / metabolism* Endosomes / metabolism Eye / growth & development* Eye / metabolism* Eye Abnormalities / genetics Eye Abnormalities / metabolism Gene Knockdown Techniques Glycoproteins / metabolism Humans Lysosomes / metabolism Molecular Sequence Data Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Photoreceptor Cells, Invertebrate / cytology Photoreceptor Cells, Invertebrate / metabolism Protein Interaction Domains and Motifs Protein Transport RNA Interference Receptors, Notch / genetics Receptors, Notch / metabolism* Sequence Homology, Amino Acid Signal Transduction Transcription Factor AP-1 / chemistry Transcription Factor AP-1 / metabolism* Transcription Factor AP-2 / chemistry Transcription Factor AP-2 / metabolism trans-Golgi Network / metabolism |
| IF | 4.573 |
| 引用数 | 8 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | actin-GAL4 GMR-GAL4 ey-GAL4 UAS-lacZ UAS-GFP 6390R-2 6390R-4 3002R-2 3002R-3 9388R-2 9388R-6 5864R-2 5864R-3 9113R-2 9113R-3 12532R-2 12532R-3 8385R-1 8385R-2 11027R-2 11027R-3 9012R-1 9012R-2 |