論文 - 詳細
| RRC ID | 21910 |
|---|---|
| 著者 | Molnar C, Casado M, López-Varea A, Cruz C, de Celis JF. |
| タイトル | Genetic annotation of gain-of-function screens using RNA interference and in situ hybridization of candidate genes in the Drosophila wing. |
| ジャーナル | Genetics |
| Abstract |
Gain-of-function screens in Drosophila are an effective method with which to identify genes that affect the development of particular structures or cell types. It has been found that a fraction of 2-10% of the genes tested, depending on the particularities of the screen, results in a discernible phenotype when overexpressed. However, it is not clear to what extent a gain-of-function phenotype generated by overexpression is informative about the normal function of the gene. Thus, very few reports attempt to correlate the loss- and overexpression phenotype for collections of genes identified in gain-of-function screens. In this work we use RNA interference and in situ hybridization to annotate a collection of 123 P-GS insertions that in combination with different Gal4 drivers affect the size and/or patterning of the wing. We identify the gene causing the overexpression phenotype by expressing, in a background of overexpression, RNA interference for the genes affected by each P-GS insertion. Then, we compare the loss and gain-of-function phenotypes obtained for each gene and relate them to its expression pattern in the wing disc. We find that 52% of genes identified by their overexpression phenotype are required during normal development. However, only in 9% of the cases analyzed was there some complementarity between the gain- and loss-of-function phenotype, suggesting that, in general, the overexpression phenotypes would not be indicative of the normal requirements of the gene. |
| 巻・号 | 192(2) |
| ページ | 741-52 |
| 公開日 | 2012-10-1 |
| DOI | 10.1534/genetics.112.143537 |
| PII | genetics.112.143537 |
| PMID | 22798488 |
| PMC | PMC3454894 |
| MeSH | Animals DNA Transposable Elements / genetics* Drosophila Proteins / genetics* Drosophila melanogaster* / genetics Drosophila melanogaster* / growth & development Drosophila melanogaster* / metabolism Gene Expression Regulation, Developmental Genetic Association Studies In Situ Hybridization Mutagenesis, Insertional / genetics Mutation Phenotype RNA Interference Receptors, Notch / genetics Receptors, Notch / metabolism Transcription Factors / genetics* Wings, Animal* / cytology Wings, Animal* / metabolism |
| IF | 4.015 |
| 引用数 | 2 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| ショウジョウバエ | 1009R-3 10473R-3 10475R-1 10496R-3 10574R-2 10777R-4 10868R-5 11290R-1 11936R-1 12051R-2 12086R-3 12112R-2 12113R-2 12138R-1 12204R-2 12296R-3 12530R-3 12960R-1 12964R-2 13112R-2 13398R-2 13681R-1 13698R-3 13892R-1 13894R-1 14291R-2 14441R-3 14508R-2 14656R-3 15150R-2 15173R-1 15525R-1 1655R-1 1715R-1 17246R-2 17390R-2 17753R-1 17836R-2 18623R-4 2048R-3 2072R-3 2186R-4 30217R-6 30444R-1 31044R-1 31044R-2 3171R-2 32085R-2 32099R-1 3593R-2 3836R-3 3841R-1 3886R-1 3940R-1 3998R-2 4012R-1 4105R-2 4159R-3 4337R-2 4399R-2 4651R-1 4799R-1 4903R-2 4924R-3 4930R-1 4943R-2 5086R-1 5180R-1 5361R-2 5585R-1 5737R-4 5838R-1 5986R-1 6392R-1 6586R-2 6588R-1 6759R-3 7425R-1 7574R-2 7832R-1 7832R-3 7865R-1 7923R-1 8097R-1 8171R-2 8281R-3 8525R-1 8834R-3 8963R-1 9021R-3 9310R-1 9314R-1 9576R-3 9591R-2 9635R-3 9668R-1 |