論文 - 詳細
| RRC ID | 21958 |
|---|---|
| 著者 | Glatter T, Schittenhelm RB, Rinner O, Roguska K, Wepf A, Jünger MA, Köhler K, Jevtov I, Choi H, Schmidt A, Nesvizhskii AI, Stocker H, Hafen E, Aebersold R, Gstaiger M. |
| タイトル | Modularity and hormone sensitivity of the Drosophila melanogaster insulin receptor/target of rapamycin interaction proteome. |
| ジャーナル | Mol Syst Biol |
| Abstract |
Genetic analysis in Drosophila melanogaster has been widely used to identify a system of genes that control cell growth in response to insulin and nutrients. Many of these genes encode components of the insulin receptor/target of rapamycin (InR/TOR) pathway. However, the biochemical context of this regulatory system is still poorly characterized in Drosophila. Here, we present the first quantitative study that systematically characterizes the modularity and hormone sensitivity of the interaction proteome underlying growth control by the dInR/TOR pathway. Applying quantitative affinity purification and mass spectrometry, we identified 97 high confidence protein interactions among 58 network components. In all, 22% of the detected interactions were regulated by insulin affecting membrane proximal as well as intracellular signaling complexes. Systematic functional analysis linked a subset of network components to the control of dTORC1 and dTORC2 activity. Furthermore, our data suggest the presence of three distinct dTOR kinase complexes, including the evolutionary conserved dTTT complex (Drosophila TOR, TELO2, TTI1). Subsequent genetic studies in flies suggest a role for dTTT in controlling cell growth via a dTORC1- and dTORC2-dependent mechanism. |
| 巻・号 | 7 |
| ページ | 547 |
| 公開日 | 2011-11-8 |
| DOI | 10.1038/msb.2011.79 |
| PII | msb201179 |
| PMID | 22068330 |
| PMC | PMC3261712 |
| MeSH | Animals Cell Line Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Drosophila melanogaster / physiology Mass Spectrometry Protein Interaction Maps Protein Kinases / genetics Protein Kinases / metabolism* Proteome / genetics Proteome / metabolism* Receptor, Insulin / genetics Receptor, Insulin / metabolism* Signal Transduction TOR Serine-Threonine Kinases Transcription Factors / metabolism |
| IF | 8.991 |
| 引用数 | 46 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 16908R-1 |